A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors

• Published in: The Journal of immunology (1950) Vol. 155; no. 5; pp. 2661 - 2667
• Main Authors: Youn, BS, Jang, IK, Broxmeyer, HE, Cooper, S, Jenkins, NA, Gilbert, DJ, Copeland, NG, Elick, TA, Fraser, MJ, Jr, Kwon, BS
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01-09-1995
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Bone Marrow - drug effects; Bone Marrow Cells; Chemokine CCL4; Chemokines, CC; Chromosome Mapping; Cloning, Molecular; Colony-Forming Units Assay; Cytokines - agonists; Cytokines - pharmacology; Hematopoietic Stem Cells - drug effects; Macrophage Inflammatory Proteins; Mice; Molecular Sequence Data; Monokines - agonists; Monokines - pharmacology
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.155.5.2661

A new member of the beta-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1 alpha. MRP-2 shows a 50.8% sequence identity at the protein level to MRP-1 and 46.3% identity to MIP-1 alpha. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the beta-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst-forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage-megakaryocyte) progenitor cells stimulated by combinations of growth factors.