The Effect of Pelargonium endlicherianum Fenzl. root extracts on formation of nanoparticles and their antimicrobial activities

• Published in: Enzyme and microbial technology Vol. 97; pp. 21 - 26
• Main Authors: Şeker Karatoprak, Gökçe, Aydin, Gamze, Altinsoy, Berrak, Altinkaynak, Cevahir, Koşar, Müberra, Ocsoy, Ismail
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-02-2017
• Subjects: Acetophenones - isolation & purification; Acetophenones - pharmacology; Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; Antimicrobial activity; Escherichia coli - drug effects; Gallic Acid - isolation & purification; Gallic Acid - pharmacology; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Microbial Sensitivity Tests; Nanotechnology; Pelargonium - chemistry; Pelargonium endlicherianum; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Roots - chemistry; Plants, Medicinal - chemistry; Pseudomonas aeruginosa - drug effects; Quercetin - isolation & purification; Quercetin - pharmacology; Silver; Silver nanoparticle; Staphylococcus epidermidis - drug effects; Antimicrobial activity; Pelargonium endlicherianum; Silver nanoparticle
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/j.enzmictec.2016.10.019

•Pelargonium endlicherianum Fenzl.•Isolation and identification.•The biosynthesis of Ag NPs.•Antibacterial activity.
Herein, we report the biosynthesis of Ag NPs, for the first time, using identified antimicrobial molecules (gallic acid+apocynin) and (gallic acid+apocynin+quercetin) from the medicinal plant Pelargonium endlicherianum Fenzl. and dramatically enhanced antimicrobial activity. We also investigate the role of each molecule on formation Ag NPs and explain the increase in the antimicrobial activity of identified molecules mediated Ag NPs. The extraction protocols, 11% ethanol and 70% methanol, resulted in identification of different constituents of gallic acid+apocynin (M1) and gallic acid+apocynin+quercetin (M2) with respective concentrations. The M1-Ag and M2-Ag NPs exhibit excellent inhibitory activities towards Gram negative bacteria; Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Gram positive bacteria; Staphylococcus epidermidis ATCC 3699 bacterial using in vitro microdilution method. The minimum inhibitory concentration (MIC) values of M1-Ag and M2-Ag NPs were determined to be 7.81 and 6.25ppm for S. epidermidis, respectively. Surprisingly, MIC value for both Ag NPs was indicated to be identical as 9. 37ppm for P. aeruginosa and E., coli.