Antibiofilm Activity of Weissella spp. and Bacillus coagulans Isolated from Equine Skin against Staphylococcus aureus

• Published in: Life (Basel, Switzerland) Vol. 12; no. 12; p. 2135
• Main Authors: Styková, Eva, Nemcová, Radomíra, Maďar, Marián, Bujňáková, Dobroslava, Mucha, Rastislav, Gancarčíková, Soňa, Requena Domenech, Francisco
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17-12-2022; MDPI
• Subjects: antibiofilm activity; Antibiotics; Antiinfectives and antibacterials; antimicrobial activity; Antimicrobial agents; Bacillus coagulans; Bacteria; Bacterial infections; Bacteriocins; Biofilms; Biosurfactants; Catalase; Dermatitis; Dispersion; Drug resistance; Horses; Hydrogen peroxide; Methicillin; Microorganisms; Pathogens; Peroxides; probiotic; Skin; Skin tests; Staphylococcus aureus; Staphylococcus infections; Surfactants; Trypsin; Weissella; Weissella spp; England; Austria; United Kingdom > UK; United States > US; Germany; skin; probiotic; Weissella spp; antimicrobial activity; Bacillus coagulans; antibiofilm activity
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life12122135

The aim of this study was to evaluate the antimicrobial and antibiofilm activity of
,
and
, isolated from equine skin, against biofilm-forming
CCM 4223 and clinical isolate methicillin-resistant
(MRSA). Non-neutralized cell-free supernatants (nnCFS) of tested skin isolates completely inhibited the growth and biofilm formation of
strains and caused dispersion of the 24 h preformed biofilm in the range of 21-90%. The majority of the pH-neutralized cell-free supernatants (nCFS) of skin isolates inhibited the biofilm formation of both
strains in the range of 20-100%. The dispersion activity of
nCFS ranged from 17 to 77% and was significantly lower than that of nnCFS, except for
3T27 against
CCM 4223. Changes in the growth of
CCM 4223 in the presence of catalase- or trypsin-treated
4/2D23 and
4/8D37 nCFS indicated the role of peroxides and/or bacteriocin in their antimicrobial activities. For the first time, the presence of the
gene, associated with biosurfactants production, was detected in
. The results of this study showed that selected isolates may have the potential for the prevention and treatment of biofilm-forming
infections.