The radioprotective effect of a new aminothiol (20-PRA)

• Published in: Brazilian journal of medical and biological research Vol. 31; no. 8; pp. 1095 - 1098
• Main Authors: Dolabela, M F, Pereira, M T, Salas, C E, Steffani, G M, Nelson, D L, Piló-Veloso, D, Lopes, M T
• Format: Journal Article
• Language: English
• Published: Brazil Associação Brasileira de Divulgação Científica 01-08-1998
• Subjects: aminothiols; Animals; antitumorigenic effect; BIOLOGY; cytotoxic effect; Humans; MEDICINE, RESEARCH & EXPERIMENTAL; Mercaptoethylamines - pharmacology; Propylamines - pharmacology; Radiation-Protective Agents - pharmacology; radioprotector; Sulfhydryl Compounds - pharmacology; Tumor Cells, Cultured - drug effects; radioprotector; antitumorigenic effect; cytotoxic effect; aminothiols
• ISSN: 0100-879X; 1414-431X; 0100-879X; 1414-431X
• DOI: 10.1590/S0100-879X1998000800010

We examined the radioprotective effect of aminothiol 2-N-propylamine-cyclo-hexanethiol (20-PRA) on a human leukemic cell line (K562) following various radiation doses (5, 7.5 and 20 Gy) using a source of 60Co gamma-rays. At 5 Gy and 1 nM 20-PRA, a substantial protective effect (58%) was seen 24 h after irradiation, followed by a decrease at 48 h (11%). At the high radiation dose (20 Gy) a low protective effect was also seen (35%). In addition, the antitumorigenic potential of 10 nM 20-PRA was shown by the inhibition of crown gall formation induced by Agrobacterium tumefaciens. The radioprotective potency of 20-PRA is 10(5)-10(6) times higher than that of the aminothiol WR-1065 (N-(2-mercaptoethyl)-1,3-diaminopropane) whose protective effect is in the 0.1 to 1.0 mM range.