Nitric Oxide Enhanced Salt Stress Tolerance in Tomato Seedlings, Involving Phytohormone Equilibrium and Photosynthesis

• Published in: International journal of molecular sciences Vol. 23; no. 9; p. 4539
• Main Authors: Wei, Lijuan, Zhang, Jing, Wei, Shouhui, Hu, Dongliang, Liu, Yayu, Feng, Li, Li, Changxia, Qi, Nana, Wang, Chunlei, Liao, Weibiao
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20-04-2022; MDPI
• Subjects: Abiotic stress; Abscisic acid; Carbon; Cell division; Chlorophyll; chlorophyll fluorescence; Enzymes; Gene expression; Genes; Hormones; Indoleacetic acid; Kinases; Lycopersicon esculentum - genetics; Metabolic pathways; Metabolism; Nitric oxide; Nitric Oxide - metabolism; Oxidative stress; Photosynthesis; photosynthetic capacity; Physiology; phytohormone; Phytohormones; Plant growth; Plant Growth Regulators - metabolism; Proteins; RNA-Seq; Roles; Salicylic acid; Salinity; Salinity tolerance; Salt Stress; Seedlings; Seedlings - genetics; Signal transduction; Sodium chloride; Sodium Chloride - metabolism; Sodium Chloride - pharmacology; Stress response; Stress, Physiological; Tomatoes; Toxicity; Transcriptomes; nitric oxide; salt stress; photosynthetic capacity; RNA-Seq; chlorophyll fluorescence; phytohormone
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23094539

Nitric oxide (NO), as a ubiquitous gas signaling molecule, modulates various physiological and biochemical processes and stress responses in plants. In our study, the NO donor nitrosoglutathione (GSNO) significantly promoted tomato seedling growth under NaCl stress, whereas NO scavenger 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide potassium (cPTIO) treatment reversed the positive effect of NO, indicating that NO plays an essential role in enhancing salt stress resistance. To explore the mechanism of NO-alleviated salt stress, the transcriptome of tomato leaves was analyzed. A total of 739 differentially expressed genes (DEGs) were identified and classified into different metabolic pathways, especially photosynthesis, plant hormone signal transduction, and carbon metabolism. Of these, approximately 16 and 9 DEGs involved in plant signal transduction and photosynthesis, respectively, were further studied. We found that GSNO increased the endogenous indoleacetic acid (IAA) and salicylic acid (SA) levels but decreased abscisic acid (ABA) and ethylene (ETH) levels under salt stress conditions. Additionally, GSNO induced increases in photosynthesis pigment content and chlorophyll fluorescence parameters under NaCl stress, thereby enhancing the photosynthetic capacity of tomato seedlings. Moreover, the effects of NO mentioned above were reversed by cPTIO. Together, the results of this study revealed that NO regulates the expression of genes related to phytohormone signal transduction and photosynthesis antenna proteins and, therefore, regulates endogenous hormonal equilibrium and enhances photosynthetic capacity, alleviating salt toxicity in tomato seedlings.