LKTA and PlpE small fragments fusion protein protect against Mannheimia haemolytica challenge

• Published in: Research in veterinary science Vol. 93; no. 3; pp. 1293 - 1300
• Main Authors: Guzmán-Brambila, Carolina, Quintero-Fabián, Saray, González-Castillo, Celia, de Obeso-Fernández del Valle, Álvaro, Flores-Samaniego, Beatriz, de la Mora, Germán, Rojas-Mayorquín, Argelia E., Ortuño-Sahagún, Daniel
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01-12-2012; Elsevier Limited
• Subjects: adverse effects; Animals; Antibodies, Bacterial; Bacterial Outer Membrane Proteins - chemistry; Bacterial Outer Membrane Proteins - immunology; Bacterial Proteins - chemistry; Bacterial Proteins - immunology; Bacterial Vaccines - immunology; bovine respiratory disease; Cattle; Cloning; Cloning, Molecular; Deoxyribonucleic acid; Disease; DNA; Enzyme-Linked Immunosorbent Assay - veterinary; feeder cattle; feedlots; Hemolysin Proteins - chemistry; Hemolysin Proteins - immunology; industry; Infections; Leukotoxin; leukotoxins; lipoproteins; Lipoproteins - chemistry; Lipoproteins - immunology; Mannheimia haemolytica; Mannheimia haemolytica - immunology; Mice; Mice, Inbred BALB C; Microbiology; outer membrane proteins; Pasteurellaceae Infections - prevention & control; Pasteurellaceae Infections - veterinary; pathogens; PlpE; Pneumonia; Proteins; Rabbits; recombinant fusion proteins; Recombinant Proteins; Respiratory bovine complex; Respiratory diseases; Sheep; Vaccine; Vaccines; Veterinary medicine; Leukotoxin; Mannheimia haemolytica; Respiratory bovine complex; Vaccine; PlpE
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2012.07.004

Bovine respiratory disease (BRD) complex is a major cause of economic losses for the cattle backgrounding and feedlot industries. Mannheimia haemolytica is considered the most important pathogen associated with this disease. Vaccines against M. haemolytica have been prepared and used for many decades, but traditional bacterins have failed to demonstrate effective protection and their use has often exacerbated disease in vaccinated animals. Thus, the BRD complex continues to exert a strong adverse effect on the health and wellbeing of stocker and feeder cattle. Therefore, generation of recombinant proteins has been helpful in formulating enhanced vaccines against M. haemolytica, which could confer better protection against BRD. In the present study, we formulated a vaccine preparation enriched with recombinant small fragments of leukotoxin A (LKTA) and outer-membrane lipoprotein (PlpE) proteins, and demonstrated its ability to generate high antibody titers in rabbits and sheep, which protected against M. haemolytica bacterial challenge in mice.