Detection of Rickettsia rickettsii and Rickettsia sp. in Blood Clots in 24 Patients from Different Municipalities of the State of São Paulo, Brazil

• Published in: Annals of the New York Academy of Sciences Vol. 1078; no. 1; pp. 260 - 262
• Main Authors: GEHRKE, FLÁVIA SOUSA, MENDES do NASCIMENTO, ELVIRA MARIA, RODRIGUES de SOUZA, ELIANA, COLOMBO, SILVIA, JACINTHO da SILVA, LUIZ, SCHUMAKER, TERESINHA TIZU SATO
• Format: Journal Article
• Language: English
• Published: Malden, USA Blackwell Publishing Inc 01-10-2006
• Subjects: Blood Coagulation; Brazil; Brazil - epidemiology; Brazilian spotted fever; DNA Primers; Humans; ompB gene; Polymerase Chain Reaction; Reproducibility of Results; Rickettsia - genetics; Rickettsia - isolation & purification; Rickettsia Infections - blood; Rickettsia Infections - epidemiology; Rickettsia rickettsii; Rickettsia rickettsii - genetics; Rickettsia rickettsii - isolation & purification; Rickettsia sp; Rocky Mountain Spotted Fever - blood; Rocky Mountain Spotted Fever - epidemiology; São Paulo; Brazil
• ISSN: 0077-8923; 1749-6632
• DOI: 10.1196/annals.1374.047

:  The authors detected Rickettsia genus organisms using shell vial and polymerase chain reaction (PCR)/sequencing analysis in blood clots in patients suspected of having Brazilian spotted fever (BSF). DNA was detected using PCR with three sets of primers to access the gltA, ompA, and ompB genes. Sequence analysis was carried out using an automatic sequencer with Bioedit® software. Seventy‐five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. To date, 46% of the samples have been sequenced.