Nematicidal activity of furanocoumarins from parsley against Meloidogyne spp

• Published in: Pest management science Vol. 71; no. 8; pp. 1099 - 1105
• Main Authors: Caboni, Pierluigi, Saba, Marco, Oplos, Chrisostomos, Aissani, Nadhem, Maxia, Andrea, Menkissoglu‐Spiroudi, Urania, Casu, Laura, Ntalli, Nikoletta
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-08-2015; Wiley Subscription Services, Inc
• Subjects: aerial parts; allelochemical; Animals; Antinematodal Agents; Arenaria; bergapten; Chromatography, Liquid; dose response; essential oil; Essential oils; exhibitions; females; Furocoumarins; galls; Herbs; intercropping; Lycopersicon esculentum; Lycopersicon esculentum - parasitology; Mass Spectrometry; Meloidogyne; Methanol; methoxsalen; nematicidal properties; Nematoda; nematode control; Nematodes; oils; Oils, Volatile - chemistry; parsley; Pest Control; pest management; Petroselinum - chemistry; Petroselinum crispum; Plant Components, Aerial - chemistry; Plant Extracts - chemistry; Plant Roots - parasitology; psoralen; Reproduction - drug effects; root-knot nematode; roots; soil; Soil amendment; soil amendments; Soil conditioners; Tomatoes; Tylenchoidea; Vegetable oils; soil amendment; allelochemical; essential oil; root-knot nematode; Petroselinum crispum
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.3890

BACKGROUND: This report describes activity against Meloidogyne spp. and chemical characterisation of the essential oil and methanol extract of Petroselinum crispum aerial parts. The study was based on the hypothesis that P. crispum could be used as an intercrop and soil amendment in tomato culture for nematode control. RESULTS: The methanol extract and the essential oil exhibited significant nematicidal activity against M. incognita, M. hapla and M. arenaria, the first being the most sensitive species, with EC₅₀ /₇₂ ₕ values of 140 ± 15 and 795 ± 125 mg L⁻¹ for the extract and oil respectively. The most abundant furanocoumarin compounds in the methanolic extract were xanthotoxin, psoralen, bergapten and oxypeucedanin; levels ranged from 1.77 to 46.04 mg kg⁻¹ wet weight. The EC₅₀ /₂₄ ₕ values of xanthotoxol, psoralen and xanthotoxin against M. incognita were 68 ± 33, 147 ± 88 and 200 ± 21 mg L⁻¹ respectively. The addition of fresh parsley paste to soil reduced the number of M. incognita females and plant galls on tomato roots; EC₅₀ values were 24.79 and 28.07 mg g⁻¹ respectively. Moreover, parsley paste enhanced tomato growth in a dose–response manner. CONCLUSIONS: Parsley exhibits promising nematicidal activity as an organic amendment and as a source of nematotoxic furanocoumarins. © 2014 Society of Chemical Industry