The number and distribution of bipolar to ganglion cell synapses in the inner plexiform layer of the anuran retina

• Published in: Visual neuroscience Vol. 13; no. 6; p. 1099
• Main Authors: Buzás, P, Jeges, S, Gábriel, R
• Format: Journal Article
• Language: English
• Published: England 01-11-1996
• Subjects: Animals; Axonal Transport; Bufo marinus; Cell Count; Female; Horseradish Peroxidase; Interneurons - physiology; Interneurons - ultrastructure; Male; Retina - physiology; Retina - ultrastructure; Retinal Ganglion Cells - physiology; Retinal Ganglion Cells - ultrastructure; Synapses - physiology; Synapses - ultrastructure; Visual Pathways - physiology; Visual Pathways - ultrastructure; Xenopus laevis
• ISSN: 0952-5238
• DOI: 10.1017/S0952523800007744

The main route of information flow through the vertebrate retina is from the photoreceptors towards the ganglion cells whose axons form the optic nerve. Bipolar cells of the frog have been so far reported to contact mostly amacrine cells and the majority of input to ganglion cells comes from the amacrines. In this study, ganglion cells of frogs from two species (Bufo marinus, Xenopus laevis) were filled retrogradely with horseradish peroxidase. After visualization of the tracer, light-microscopic cross sections showed massive labeling of the somata in the ganglion cell layer as well as their dendrites in the inner plexiform layer. In cross sections, bipolar output and ganglion cell input synapses were counted in the electron microscope. Each synapse was assigned to one of the five equal sublayers (SLs) of the inner plexiform layer. In both species, bipolar cells were most often seen to form their characteristic synaptic dyads with two amacrine cells. In some cases, however, the dyads were directed to one amacrine and one ganglion cell dendrite. This type of synapse was unevenly distributed within the inner plexiform layer with the highest occurrence in SL2 both in Bufo and Xenopus. In addition, SL4 contained also a high number of this type of synapse in Xenopus. In both species, we found no or few bipolar to ganglion cell synapses in the marginal sublayers (SLs 1 and 5). In Xenopus, 22% of the bipolar cell output synapses went onto ganglion cells, whereas in Bufo this was only 10%. We conclude that direct bipolar to ganglion cell information transfer exists also in frogs although its occurrence is not as obvious and regular as in mammals. The characteristic distribution of these synapses, however, suggests that specific type of the bipolar and ganglion cells participate in this process. These contacts may play a role in the formation of simple ganglion cell receptive fields.