Simple and cleaner system of silver nanoparticle synthesis using kenaf seed and revealing its anticancer and antimicrobial potential

• Published in: Nanotechnology Vol. 31; no. 26; p. 265101
• Main Authors: Adnan, Md, Obyedul Kalam Azad, Md, Madhusudhan, Alle, Saravanakumar, Kandasamy, Hu, Xiaowen, Wang, Myeong-Hyeon, Ha, Cho Dong
• Format: Journal Article
• Language: English
• Published: England 09-04-2020
• Subjects: A549 Cells; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Cell Proliferation - drug effects; Cell Survival - drug effects; Drug Stability; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Green Chemistry Technology; Hibiscus - chemistry; Humans; Metal Nanoparticles; Mice; Microbial Sensitivity Tests; NIH 3T3 Cells; Seeds - chemistry; Silver - chemistry; Silver - pharmacology
• ISSN: 1361-6528
• DOI: 10.1088/1361-6528/ab7d72

The clean and eco-friendly synthesis of silver nanoparticles (AgNPs) has provided promising characteristics with impressive biomedical related potential. Here, we have employed a green process for the synthesis of AgNPs using kenaf seed (KS) extract as a bilateral mediator for reducing and capping of Ag
ions under hydrothermal condition. The synthesis pathways, such as varying amounts of KS, Ag ion concentration and autoclaving time were optimized. The manifestation of a strong absorption peak from 420-430 nm in UV-vis spectroscopy indicated the successful synthesis of KS@AgNPs. Fourier transform infrared spectroscopy confirmed the presence of hydroxyl and carbonyl functionalities involved in the reduction and stabilization of Ag
ions. Furthermore, transmission electron microscopy revealed that the KS@AgNPs are spherical in shape having a size around 7-11 nm, whereas high-quality crystals were evidenced by x-ray diffraction analysis. Moreover, inductively coupled plasma-optical emission spectrometry revealed that 19.6 μg l
of Ag
ions were released from the KS@AgNPs. In cell line studies, KS@AgNPs at a higher dose were shown to be non-toxic to the healthy (NIH3T3) cells, while strong anti-proliferative response was found in the case of lung cancer (A549) cells. Furthermore, a significant zone of inhibition was observed for both Gram-positive and Gram-negative microorganisms, and a combination of KS@AgNPs with ampicillin revealed a notable synergistic anti-pathogenic effect. Overall, our study proved the potentiality of KS as an efficient bio-resource for the synthesis of AgNPs and also its original feature as an anti-cancer and antimicrobial agent.