Separation and Determination of Water Soluble Active Components in Salvia miltiorrhiza Bunge and Its Pharmaceutical Preparations by Capillary Zone Electrophoresis with Diode Array Detection

• Published in: Journal of liquid chromatography & related technologies Vol. 30; no. 19; pp. 2819 - 2833
• Main Authors: Qiao, Chengdong, Zhao, Liang, Jiang, Shengxiang, Song, Pingshun
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01-01-2007; Taylor & Francis
• Subjects: Biological and medical sciences; Capillary zone electrophoresis; Danshensu; General pharmacology; Medical sciences; Pharmaceutical preparations; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Separation; Asia; China; Danshensu; Chemical analysis; Zone electrophoresis; Separation; Phenolic acid; Diphenols; Medicinal plant; Folk medicine; Dicotyledones; Angiospermae; Diode array; Tablet; Pharmaceutical preparations; Capillary zone electrophoresis; Capillary electrophoresis; Pharmacognosy; Oral form; Aldehydophenols; Water soluble compound; Salvia miltiorrhiza; Ultraviolet detector; Labiatae; Quality control; Plant origin; Dosage form; Active ingredient; Spermatophyta; Pill
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826070701588513

A novel, simple, and rapid method is developed for separating water soluble active components in Salvia miltiorrhiza Bunge and its pharmaceutical preparations by capillary zone electrophoresis with a diode array detector within 11 min under the optimal conditions. The repeatability (defined as relative standard deviation, RSD) was less than 4.45% with peak area evaluation and 2.13% with migration time evaluation. Regression equations revealed linear relationships (r: 0.9964-0.9987) between the peak area of each analyte and the concentration. The detection limits (S/N=3) of Protocatechuic Aldehyde, Danshensu, and Protocatechuic Acid were 0.46, 0.62, and 0.28 µg/mL, respectively. The proposed method has been satisfactorily applied to the analysis of water soluble active components in Salvia miltiorrhiza Bunge and its pharmaceutical preparations with recoveries in the range of 94%-101%.