A reproducible workflow for assembling the mitochondrial genome of Acheta domesticus (Orthoptera: Gryllidae)

• Published in: Ecology and evolution Vol. 14; no. 7; pp. e11696 - n/a
• Main Authors: Homchan, Somjit, Kutanan, Wibhu, Gupta, Yash Munnalal
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01-07-2024; John Wiley and Sons Inc; Wiley
• Subjects: Acheta domesticus; Annotations; Assembling; Bats; Bayesian analysis; Biodiversity Ecology; Breeding; Clustering; Entomology; Evolutionary genetics; Food security; galaxy workflow; Gene sequencing; Genes; Genetics; Genetics Notes; Genomes; Genomics; house cricket; Identification; Insects; Machine learning; Mitochondrial DNA; mitochondrial genome; Morphology; Nucleotides; Phylogenetics; phylogeny; Python; rRNA; Transfer RNA; tRNA; Workflow; Europe; house cricket; mitochondrial genome; phylogeny; galaxy workflow; insects
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.11696

In this study, we report the assembly and annotation of the mitochondrial genome (mitogenome) of Acheta domesticus from breeding facility, a species commonly known as the house cricket. This species is considered to be an important edible cricket. The mitogenome was assembled using a reproducible protocol implemented on the Galaxy Europe Server, which involved uploading paired‐end fastq reads for bioinformatic analysis. The resulting mitogenome is 15,784 base pairs in length and has a GC content of 29.05%. The nucleotide composition of this mitogenome is similar to that of other insect mitogenomes, with A, T, C, and G nucleotides comprising 39.2%, 31.7%, 19.6%, and 9.5% of the mitogenome, respectively. The gene organization of the A. domesticus mitogenome is identical to that of other cricket species. The mitogenome consists of 37 genes, including 13 protein‐coding genes, 22 tRNA genes, and two rRNA genes. The congruence between PCA and Bayesian evolutionary tree analysis in clustering the divergent A. domesticus sequences highlights these genomes as candidates for further study to elucidate their distinct features and evolutionary history.
This study presents the comprehensive assembly and analysis of the complete mitochondrial genome of the economically important house cricket, Acheta domesticus. The high‐quality mitogenomic data obtained through our robust bioinformatic workflow serve as an invaluable resource for precise DNA barcoding, species identification, and evolutionary studies within the order Orthoptera. The comparative genomic analysis and phylogenetic insights derived from this research shed light on the evolutionary relationships and genetic diversity of A. domesticus and related cricket species.