HLA-DR Expression on Monocyte Subsets in Critically Ill Children

• Published in: The Pediatric infectious disease journal Vol. 37; no. 10; pp. 1034 - 1040
• Main Authors: Boeddha, Navin P, Kerklaan, Dorian, Dunbar, Albert, van Puffelen, Esther, Nagtzaam, Nicole M A, Vanhorebeek, Ilse, Van den Berghe, Greet, Hazelzet, Jan A, Joosten, Koen F, Verbruggen, Sascha C, Dik, Willem A, Driessen, Gertjan J
• Format: Journal Article
• Language: English
• Published: United States Copyright Wolters Kluwer Health, Inc. All rights reserved 01-10-2018
• ISSN: 0891-3668; 1532-0987
• DOI: 10.1097/INF.0000000000001990

BACKGROUND:To longitudinally study blood monocyte subset distribution and human leukocyte antigen-DR (HLA-DR) expression on monocyte subsets in children with sepsis, post-surgery and trauma in relation to nosocomial infections and mortality.
METHODS:In 37 healthy children and 37 critically ill children (12 sepsis, 11 post-surgery, 10 trauma and 4 admitted for other reasons)—participating in a randomized controlled trial on early versus late initiation of parenteral nutrition—monocyte subset distribution and HLA-DR expression on monocyte subsets were measured by flow cytometry upon admission and on days 2, 3 and 4 of pediatric intensive care unit (PICU) stay.
RESULTS:Upon PICU admission, critically ill children had a higher proportion of classical monocytes (CD14++CD16−) than healthy children [PICU 95% (interquartile range [IQR] 88%–98%); controls, 87% (IQR 85%–90%), P < 0.001]. HLA-DR expression was significantly decreased within all monocyte subsets and at all time points, being most manifest on classical monocytes and in patients with sepsis. Percentage of HLA-DR expressing classical monocytes [upon PICU admission 67% (IQR 44%–88%); controls 95% (IQR 92%–98%), P < 0.001], as well as the HLA-DR mean fluorescence intensity [upon PICU admission 3219 (IQR 2650–4211); controls 6545 (IQR 5558–7647), P < 0.001], decreased during PICU stay. Patients who developed nosocomial infections (n = 13) or who died (n = 6) had lower HLA-DR expression on classical monocytes at day 2 (P = 0.002) and day 3 (P = 0.04), respectively.
CONCLUSIONS:Monocytic HLA-DR expression decreased during PICU stay and was lower compared with controls on all examined time points, especially on classical monocytes and in children admitted for sepsis. Low HLA-DR expression on classical monocytes was associated with nosocomial infections and death.