Fine mapping and candidate gene analysis of the quantitative trait locus gw8.1 associated with grain length in rice

• Published in: Genes & genomics Vol. 40; no. 4; pp. 389 - 397
• Main Authors: Kang, Yun-Joo, Shim, Kyu-Chan, Lee, Hyun-Sook, Jeon, Yun-A, Kim, Sun-Ha, Kang, Ju-Won, Yun, Yeo-Tae, Park, In-Kyu, Ahn, Sang-Nag
• Format: Journal Article
• Language: English
• Published: Seoul The Genetics Society of Korea 01-04-2018; Springer Nature B.V; 한국유전학회
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Chromosome 8; Chromosome Mapping - methods; Crosses, Genetic; Edible Grain - genetics; Gene expression; Gene mapping; Genes, Plant; Genetic Association Studies; Human Genetics; Kinases; Life Sciences; Microbial Genetics and Genomics; Mutation; Oryza - genetics; Oryza rufipogon; Phenotype; Plant Genetics and Genomics; Quantitative Trait Loci; Research Article; Ribonucleic acid; RNA; Scanning electron microscopy; 생물학; Quantitative trait loci; Grain weight; Near isogenic line; Rice; Oryza rufipogon
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-017-0640-6

A quantitative trait locus (QTL)
gw8.1
was detected in the population derived from a cross between the elite
japonica
cultivar, ‘Hwaseong’ and
Oryza rufipogon
(IRGC 105491). Near isogenic lines (NILs) harboring the
O. rufipogon
segment on chromosome 8 showed increased grain length and weight compared to those of the recurrent parent, Hwaseong. This QTL was mapped to a 175.3-kb region containing 28 genes, of which four were considered as candidates based on the presence of mutations in their coding regions and as per the RNA expression pattern during the inflorescence stage. Leaves and panicles obtained from plants harvested 5 days after heading showed differences in gene expression between Hwaseong and
gw8.1
-NILs. Most genes were upregulated in
O. rufipogon
and
gw8.1
-NIL than in Hwaseong. Scanning electron microscopy analysis of the lemma inner epidermal cells indicated that cell length was higher in
gw8.1
NIL than in Hwaseong, indicating that
gw8.1
might regulate cell elongation. Among the candidate genes, LOC_Os08g34380 encoding a putative receptor-like kinase and LOC_Os08g34550 encoding putative RING-H2 finger protein were considered as possible candidates based on their functional similarity.