LC-MS/MS-based chemical profiling of water extracts of Moringa oleifera leaves and pharmacokinetics of their major constituents in rat plasma

• Published in: Food Chemistry: X Vol. 23; p. 101585
• Main Authors: Wang, Jiahong, Du, Yiyang, Jiang, Li, Li, Jiahe, Yu, Bing, Ren, Chuang, Yan, Tingxu, Jia, Ying, He, Bosai
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 30-10-2024; Elsevier
• Subjects: Bioavailability; Method validation; Organic acids; Polyphenolic compounds; Polyphenolic compounds; Bioavailability; Method validation; Organic acids
• ISSN: 2590-1575; 2590-1575
• DOI: 10.1016/j.fochx.2024.101585

Moringa oleifera leaves (MOL) are native to India and have high biological activities. To better understand the basic pharmacodynamic materials, the chemical components in MOL and their pharmacokinetic properties were studied and quantitated using UPLC-Q-Exactive Orbitrap-MS. Forty-two compounds were identified, including phenolic acids and their derivatives, flavonoids, isothiocyanates, nucleosides, alkaloids, and other compounds. Two phenolic acids and six flavonoids were studied for their pharmacokinetic properties using UHPLC-MS/MS. Precision, accuracy, stability, matrix effects, and extraction recovery were verified. All substances that were measured reached their maximum within 0.5 h. Vicenin-2 had a high peak concentration and bioavailability. Kaempferol-3-O-rutinoside had a longer biological half-life than other components. The results from this study provide the data basis for subsequent comprehensive qualitative evaluation and potential MOL use in clinical applications.
•Development of a sensitive, efficient, and precise UHPLC-MS/MS method for MOL analysis in plasma samples•Identification of a total of 43 compounds in MOL by UPLC-Q-Exactive Orbitrap-MS analysis.•Investigation of pharmacokinetic behavior of 8 MOL components in rats using UHPLC-MS/MS.