Antioxidant activities of Ganoderma lucidum polysaccharides and their role on DNA damage in mice induced by cobalt-60 gamma-irradiation

• Published in: Food and chemical toxicology Vol. 50; no. 2; pp. 303 - 309
• Main Authors: Zhao, Wei, Jiang, Xuejun, Deng, Wenwen, Lai, Yanhao, Wu, Mei, Zhang, Zunzhen
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-02-2012; Elsevier
• Subjects: adverse effects; animal models; Animals; Antioxidant; antioxidant activity; Antioxidants - administration & dosage; Antioxidants - chemistry; Antioxidants - pharmacology; Biological and medical sciences; body weight; bone marrow; Chemical and industrial products toxicology. Toxic occupational diseases; Cobalt Radioisotopes - toxicity; DNA; DNA damage; DNA Damage - drug effects; DNA Damage - radiation effects; dose response; Dose-Response Relationship, Drug; Female; gamma radiation; Ganoderma - chemistry; Ganoderma lucidum; Ganoderma lucidum polysaccharides; glutathione peroxidase; leukocyte count; leukocytes; Leukocytes - drug effects; Leukocytes - radiation effects; Lipid Peroxidation; malondialdehyde; Medical sciences; Metals and various inorganic compounds; Mice; Mice, Inbred ICR; polysaccharides; Polysaccharides - administration & dosage; Polysaccharides - chemistry; Polysaccharides - pharmacology; Radioprotection; Specific Pathogen-Free Organisms; superoxide dismutase; Toxicology; Vitamin B 12 - toxicity; MPCE; MN; DNA damage; Lipid peroxidation; IR; SOD; OTM; GLP; MDA; Ganoderma lucidum polysaccharides; Antioxidant; Radioprotection; GPx; LMPA; PCE; NMPA; ROS; WBC; 60Co; Group VIII metal; Lipids; Cobalt; Basidiomycota; Fungi; Ionizing irradiation; Gamma irradiation; Carbohydrate; Nucleic acid; Ganoderma; Peroxidation; Biochemical compound; Rodentia; Oside; Transition metal; Biological activity; Vertebrata; Mammalia; Mouse; Animal; DNA; Lesion; Polysaccharide
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2011.10.071

► The radio-protective activity of Ganoderma lucidum polysaccharides (GLP) from sporoderm-broken spore powder was firstly studied. ► GLP show protective effects on DNA damage and micronuclei induction in mice induced by gamma-irradiation. ► GLP enable to increase antioxidant activities, decrease lipid peroxidation product in mice induced by gamma-irradiation.
In this study, the radio-protective effects of Ganoderma lucidum polysaccharides (GLP) were investigated in a mouse animal model exposed to 60Co gamma-irradiation. Each of three batches of mice were divided into five groups (negative control, positive gamma irradiated control, and low, middle and high dosage GLP groups). Different batches of animals were used to evaluate the impact of GLP on peripheral white blood cell count, immune organ index; DNA damage, lipid peroxidation; micronuclei formation, and nucleated cell count in bone marrow induced by 60Co gamma-irradiation. DNA strand-break and micronuclei frequency were significantly reduced and glutathione peroxidase activity and nucleated cell count in bone marrow were significantly increased by GLP treatment in a dose-dependent manner. GLP intervention also increased the activity of superoxide dismutase and decreased the level of malondialdehyde in middle and high GLP treatment groups. No adverse effects were observed on peripheral white blood cells and immune organ or body weight in either the control groups or GLP treated gamma exposed mice. These findings suggest that GLP possesses marked antioxidant capacity which plays an important role in the prevention of radiation damage in mice induced by 60Co gamma-irradiation.