Anti-T cell receptor antibodies fail to inhibit specific lysis by CTL clones but activate lytic activity for irrelevant targets

• Published in: The Journal of immunology (1950) Vol. 138; no. 4; pp. 1221 - 1228
• Main Authors: Henkel, TJ, Braciale, VL, Braciale, TJ
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15-02-1987; American Association of Immunologists
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; Biological and medical sciences; Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others); Cytotoxicity, Immunologic; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Immunological reactions in vitro; Interferon-gamma - secretion; Mice; Mice, Inbred Strains - immunology; Orthomyxoviridae - immunology; Receptors, Antigen, T-Cell - immunology; Receptors, Antigen, T-Cell - physiology; Receptors, Fc - immunology; T-Lymphocytes, Cytotoxic - immunology; T-Lymphocytes, Cytotoxic - secretion; Rodentia; Orthomyxoviridae; Cytotoxicity; Cellular immunity; Monoclonal antibody; In vitro; Influenzavirus; Virus; Antigen; Vertebrata; Membrane receptor; Specificity; Mammalia; Mouse; Animal; T-Lymphocyte; Clone
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.138.4.1221

In this report, we describe the functional effects of anti-T cell receptor antibodies on a panel of MHC-restricted, influenza virus-specific CTL clones. Approximately 25 to 30% of these clones are recognized by KJ16-133, an anti-T cell receptor monoclonal antibody presumably specific for products of the V beta 8 gene family, and an antibody with similar specificity, F23.1. In contrast to most previous reports, both KJ16-133 and F23.1, over a wide range of antibody concentrations, fail to inhibit the antigen-specific effector function of these CTL. Instead, the antibodies activate the CTL to kill without regard for the MHC haplotype of the target cells or the presence of the appropriate viral antigen. This anti-T cell receptor antibody-induced cytolysis by our clones does not appear to be mediated by Fc receptors on target cells. Nuclear destruction of target cells as a result of antibody-induced lysis suggests that it occurs via a mechanism similar to antigen-specific lysis by CTL. In addition, both soluble bivalent F23.1 and F23.1 coupled-Sepharose beads are able to induce the secretion of interferon-gamma from these CTL clones.