A partial physical map for the chromosome of alkalophilic Bacillus sp. strain C-125

• Published in: Journal of general microbiology Vol. 139; no. 4; pp. 661 - 667
• Main Authors: Sutherland, Katharine J, Hashimoto, Michizane, Kudo, Toshiaki, Horikoshi, Koki
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01-04-1993
• Subjects: Adaptation, Physiological; Bacillus; Bacillus - genetics; Bacillus - growth & development; Bacillus - physiology; Base Sequence; Chromosomes, Bacterial; DNA Restriction Enzymes; DNA, Bacterial - genetics; Hydrogen-Ion Concentration; Restriction Mapping
• ISSN: 0022-1287
• DOI: 10.1099/00221287-139-4-661

The RIKEN Institute and JRDC, Hirosawa 2–1, Wako-shi, Saitama, 351–01, Japan
* Author for correspondence
ABSTRACT
Summary: Bacillus sp. strain C-125 has been chosen as a model alkalophilic bacterium to understand how adaptation to growth at high pH is achieved. To aid genetic analysis, we have started characterization of its genome. By using the two infrequently-cutting restriction endonucleases, Asc I and Sse 8387I, in conjunction with pulsed-field electrophoretic techniques, the size of the genome was found to be 3.7 Mb. Southern blot analysis of single, double and partial digests of Bacillus sp. strain C-125 DNA, using Asc I-linking clones, gene probes and purified Bacillus sp. strain C-125 restriction fragments, allowed a putative chromosome map to be constructed.
Present address: Department of Molecular Medicine, Collier Building, Royal Postgraduate School of Medicine, DuCane Road, London, W12 0NN, UK.