Single-cell transcriptomic analysis of immune cell dynamics in the healthy human endometrium

• Published in: Biochemistry and biophysics reports Vol. 39; p. 101802
• Main Authors: Chen, Kaixing, Yu, Qiaoni, Sha, Qing, Wang, Junyu, Fang, Jingwen, Li, Xin, Shen, Xiaokun, Fu, Binqing, Guo, Chuang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-09-2024; Elsevier
• Subjects: Cell-cell interactions; Endometrium; Immune cells; Reproductive cycle; Reproductive diseases; Single-cell RNA sequencing; Cell-cell interactions; Immune cells; Single-cell RNA sequencing; Reproductive cycle; Endometrium; Reproductive diseases
• ISSN: 2405-5808; 2405-5808
• DOI: 10.1016/j.bbrep.2024.101802

The microenvironment of the endometrial immune system is crucial to the success of placental implantation and healthy pregnancy. However, the functionalities of immune cells across various stages of the reproductive cycle have yet to be fully comprehended. To address this, we conducted advanced bioinformatic analysis on 230,049 high-quality single-cell transcriptomes from healthy endometrial samples obtained during the proliferative, secretory, early pregnancy, and late pregnancy stages. Our investigation has unveiled that proliferative natural killer (NK) cells, a potential source of endometrial NK cells, exhibit the most robust proliferative and differentiation potential during non-pregnant stages. We have also identified similar differentiation trajectories of NK cells originating from proliferative NK cells across four stages. Notably, during early pregnancy, NK cells demonstrate the highest oxidative phosphorylation metabolism activity, and, in conjunction with macrophages and T cells, exhibit the strongest type II interferon response. With spatial transcriptome data, we have discerned that the most robust immune-non-immune interactions are associated with the promotion and inhibition of cell proliferation, differentiation and migration during four stages. Furthermore, we have compiled lists of stage-specific risk genes implicated in reproductive diseases, which hold promise as potential disease biomarkers. Our study provides insights into the dynamics of the endometrial immune microenvironment during different reproductive cycle stages, thus serving as a reference for detecting pathological changes during pregnancy.
•An integrated single-cell atlas of healthy human endometrial microenvironment across reproductive cycle.•An intertemporal trajectory of differentiation from proliferative NK cells to other NK cell subsets, especially the NK1 cells.•We identified stage-specific interactions related to cell proliferation, decidualization, and migration.•We identified a list of stage-specific risk genes for each cell type associated with five reproductive diseases.