Analysis of Expressed Sequence Tags (ESTs) Collected from the Inflorescence of Chrysanthemum

• Published in: Plant molecular biology reporter Vol. 27; no. 4; pp. 503 - 510
• Main Authors: Chen, Sumei, Miao, Hengbin, Chen, Fadi, Jiang, Beibei, Lu, Jungang, Fang, Weimin
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01-12-2009; Springer-Verlag; Springer Nature B.V
• Subjects: Bioinformatics; Biomedical and Life Sciences; Biosynthesis; Cell walls; Chrysanthemum; Complementary DNA; Deoxyribonucleic acid; DNA; DNA libraries; Expressed sequence tags; flowering; Gene sequencing; Genes; Homology; inflorescences; Life Sciences; Localization; Metabolism; Metabolomics; molecular cloning; Plant Breeding/Biotechnology; Plant Sciences; Protein biosynthesis; Protein turnover; Proteomics; sequence analysis; Tags; Transcription; Expressed sequence tags (ESTs); Chrysanthemum; Inflorescence; Transcripts
• ISSN: 0735-9640; 1572-9818
• DOI: 10.1007/s11105-009-0103-6

The expressed sequence tags (ESTs) described in this report were obtained from the inflorescence of chrysanthemum. A complementary DNA (cDNA) library was constructed from the inflorescence of the anemone-type chrysanthemum ‘Zhongshanzigui'. In total, 7,307 cDNA clones were sequenced, representing 4,563 unique sequences and consisting of 3,567 singletons and 996 contigs. Comparison to the GenBank nonredundant (nr) database revealed 57.2% (2,608/4,563) chrysanthemum sequences with homology to genes of known function of other organisms. Approximately 26.67% (1,217/4,563) of the unigenes were clustered into 23 categories by the clusters of orthologous group analysis: Most of the identified transcripts were genes related to metabolism, subcellular localization, protein biosynthesis, and cell wall structure. The ESTs presented here will be a valuable addition to floral development transcriptional database.