CircRNA-1926 Promotes the Differentiation of Goat SHF Stem Cells into Hair Follicle Lineage by miR-148a/b-3p/CDK19 Axis

• Published in: Animals (Basel) Vol. 10; no. 9; p. 1552
• Main Authors: Yin, Rong H., Zhao, Su J., Jiao, Qian, Wang, Ze Y., Bai, Man, Fan, Yi X., Zhu, Yu B., Bai, Wen L.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 02-09-2020; MDPI
• Subjects: Binding sites; bulge; Cashmere; Cell differentiation; circular RNA; Closed loops; Differentiation; Gene expression; Genetic aspects; Genomes; Goats; Hair; induced differentiation; Keratin; Methods; methylation; MicroRNA; MicroRNAs; Non-coding RNA; Physiological aspects; post-transcriptional; RNA sequencing; siRNA; Skin; Stem cells; Superhigh frequencies; China; United States > US
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani10091552

Circular RNAs (CircRNAs) are a type of non-coding RNAs, which contain a covalently closed loop structure without 5′ to 3′ free ends. CircRNAs play essential roles in the regeneration of secondary hair follicle (SHF) and cashmere growth in goats. CircRNA-1926 was previously identified in SHF of cashmere goats, but its potential roles are unclear. In this study, we confirmed the expression of circRNA-1926 in SHF bulge of nine cashmere goats with a significantly higher level at anagen than that of telogen. Through the use of both overexpression and siRNA interference, we showed that circRNA-1926 promoted the differentiation of SHF stem cell into hair follicle lineage in cashmere goats which was evaluated via indictor genes Keratin 7 and Keratin 17. Using RNA pull-down, we found that circRNA-1926 bound with miR-148a/b-3p. Additionally, our data indicated that circRNA-1926 promoted the expression of the CDK19 gene. Using dual-luciferase reporter assays, it was revealed that circRNA-1926 positively regulated the CDK19 expression through miR-148a/b-3p. The results from this study demonstrated that circRNA-1926 contributes the differentiation of SHF stem cells into hair follicle lineages in cashmere goats via sponging miR-148a/b-3p to enhance CDK19 expression.