Performance of a perforated rotating disc contactor in the continuous extraction of a protein using the PEG–cashew-nut tree gum aqueous two-phase system

• Published in: Biochemical engineering journal Vol. 16; no. 3; pp. 221 - 227
• Main Authors: Sarubbo, Leonie A, Oliveira, Luciana A, Porto, Ana Lúcia F, Lima-Filho, José Luiz, Campos-takaki, Galba Maria, Tambourgi, Elias B
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01-12-2003; Amsterdam Elsevier Science; New York, NY
• Subjects: Aqueous two-phase; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Liquid–liquid extraction; Mass transfer; Perforated rotating disc contactor; Separation; Liquid–liquid extraction; Aqueous two-phase; Separation; Perforated rotating disc contactor; Mass transfer; Performance evaluation; Protein; Continuous process; Ethylene oxide polymer; Liquid-liquid extraction; Biphasic system; Contactor; Rotating disk; Extraction
• ISSN: 1369-703X; 1873-295X
• DOI: 10.1016/S1369-703X(03)00023-8

The characterisation of bovine serum albumin (BSA) mass transfer mechanisms in a perforated rotating disc contactor (PRDC) using an aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and a polysaccharide, the cashew-nut tree gum, was described. The PEG-rich phase was used as the dispersed phase and protein transfer took place from the dispersed to the continuous phase. Studies of the effect of dispersed phase velocity, system composition and disc rotation speed on either protein mass transfer coefficients or column hold-up showed that the dispersed phase hold-up and the volumetric mass transfer coefficient increased with increasing the dispersed phase velocity and disc rotation speed.