Protective effect of steamed American ginseng ( Panax quinquefolius L.) on V79-4 cells induced by oxidative stress

• Published in: Journal of ethnopharmacology Vol. 111; no. 3; pp. 443 - 450
• Main Authors: Kim, Kyung Tack, Yoo, Kyung Mi, Lee, Jin Wook, Eom, Seok Hyun, Hwang, In Kyeong, Lee, Chang Y.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 22-05-2007; Elsevier
• Subjects: American ginseng ( Panax quinquefolius L.); Animals; Antioxidant capacity; antioxidants; Antioxidants - administration & dosage; Antioxidants - chemistry; Antioxidants - pharmacology; Biological and medical sciences; Biphenyl Compounds; CAT; catalase; Catalase - drug effects; Cell Line; Cell Survival - drug effects; chemical constituents of plants; Cricetinae; Dose-Response Relationship, Drug; enzyme activity; Free Radical Scavengers - administration & dosage; Free Radical Scavengers - pharmacology; Free Radicals - metabolism; General pharmacology; Ginsenosides; Ginsenosides - isolation & purification; Ginsenosides - pharmacology; Heated; Hot Temperature; Hydrogen Peroxide; Lipid Peroxidation - drug effects; Medical sciences; medicinal plants; medicinal properties; oxidative stress; Oxidative Stress - drug effects; Panax - chemistry; Panax quinquefolius; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; phenolic compounds; Phenols - isolation & purification; Phenols - pharmacology; Phytotherapy; Picrates; plant extracts; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Roots; Plants, Medicinal - chemistry; roots; SOD; superoxide dismutase; Superoxide Dismutase - drug effects; Total phenolic contents; V79-4 cells; Asia; China; VCEAC; Total phenolic contents; AAPH; American ginseng ( Panax quinquefolius L.); Antioxidant capacity; SOD; GAE; Ginsenosides; Heated; ARG; CAT; ABTS; V79-4 cells; Steroid; Oxidative stress; Panax ginseng; Medicinal plant; Saponin; Prevention; Folk medicine; Dicotyledones; Angiospermae; Glycoside; Araliaceae; Panax quinquefolius; American ginseng (Panax quinquefolius L.); Terpenoid; Pharmacognosy; Antioxidant; In vitro; Animal; Content; Plant origin; Triterpene; Spermatophyta
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.01.004

Heat-processed Asian ginseng roots (
Panax ginseng C.A. Meyer), also known as “red ginseng” in Asia, are reported to have more bioactivity than the no-processed white ginseng roots. Therefore, American fresh ginseng roots (
Panax quinquefolius L.) were processed to the red ginseng and examined changes in bioactivity during heating process. The fresh America ginseng roots were steamed at 100
°C for 30, 60, 90 and 120
min, and their bioactivities were examined by analyzing the content of ginsenosides and total phenolics, and measuring DPPH and superoxide radical scavenging acivity and their protective effects on V79-4 cells viability and lipid peroxidation. The heating treatment proportionally increased total ginsenosides (4.97%, w/w) content compared with white ginseng (3.27%) and total phenolics from 444.5
mg GAE/100
g to 489.6–574.2
mg GAE/100
g. The antioxidant activity also increased from 285
mg/100
g (vitamin C equivalent) to 353–487
mg/100
g. Heated ginseng showed high levels of DPPH radical scavenging activity (59.5–88.5%) and the high level of superoxide radical scavenging activity (44.2–90.9%). The heated ginseng protected cell viability against H
2O
2-induced oxidative damage, and enhanced the activities of superoxide dismutase and catalase by dose dependently in V79-4 cells.