Effects of miR-306 Perturbation on Life Parameters in the English Grain Aphid, Sitobion avenae (Homoptera: Aphididae)

• Published in: International journal of molecular sciences Vol. 25; no. 11; p. 5680
• Main Authors: Wu, Linyuan, Wei, Guohua, Yan, Yi, Zhou, Xuguo, Zhu, Xun, Zhang, Yunhui, Li, Xiangrui
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23-05-2024; MDPI
• Subjects: Adults; Age; Animals; Aphididae; Aphids - genetics; Aphids - physiology; Biological control; Female; Fertility - genetics; Gene expression; Genes; growth and development; Insects; Longevity - genetics; Male; MicroRNAs; MicroRNAs - genetics; miR-306; Mortality; Nanomaterials; Pest control; Pesticides; Pests; Population growth; population prediction; Sitobion avenae; two-sex life table; United Kingdom; China; population prediction; two-sex life table; growth and development; Sitobion avenae; miR-306
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25115680

MicroRNAs (miRNA) play a vital role in insects' growth and development and have significant potential value in pest control. Previously, we identified miR-306 from small RNA libraries within the English grain aphid,
, a devasting insect pest for wheat. miR-306 not only involves in wing morphogenesis, but also is critically important for aphid survival. Its specific impacts on the life history traits, however, remain unclear. Here, we evaluate the impact of miR-306 perturbation on
.
populations using a two-sex life table approach. This comprehensive analysis revealed that miR-306 perturbation significantly prolongs the developmental stages (9.64% and 8.20%) and adult longevity of
.
, while decreasing pre-adult survival rate (41.45% and 38.74%) and slightly reducing average fecundity (5.80% and 13.05%). Overall, miR-306 perturbation negatively affects the life table parameters of the aphid population. The population prediction models show a significant decline in the aphid population 60 days post interference, compared to the control groups (98.14% and 97.76%). Our findings highlight the detrimental effects of miR-306 perturbation on
.
population growth and suggest potential candidate genes for the development of RNAi-based biopesticides targeted specifically at this pest species.