Overexpression of Human Lipoprotein Lipase Increases Hormone-Sensitive Lipase Activity in Adipose Tissue of Mice

• Published in: Biochemical and biophysical research communications Vol. 211; no. 3; pp. 761 - 766
• Main Authors: Shimada, M., Ishibashi, S., Yamamoto, K., Kawamura, M., Watanabe, Y., Gotoda, T., Harada, K., Inaba, T., Ohsuga, J., Yazaki, Y., Yamada, N.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26-06-1995
• Subjects: Adipose Tissue - metabolism; Adipose Tissue, Brown - metabolism; Animals; Blotting, Northern; Body Composition; Body Weight; Humans; Lipoprotein Lipase - biosynthesis; Lipoprotein Lipase - genetics; Mice; Mice, Transgenic; Muscle, Skeletal - metabolism; Oxygen Consumption; Recombinant Proteins - biosynthesis; RNA, Messenger - analysis; Sterol Esterase - biosynthesis; Sterol Esterase - genetics; Triolein - metabolism
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1995.1878

An elevation of lipoprotein lipase (LPL) activity in adipose tissue is considered a possible cause of obesity. However, transgenic mice that overexpress the human LPL gene showed no increase in fat deposition as compared with controls. In the present study, we investigated effects of LPL on fat accumulation. Respiratory quotients and uptake of [3H] triolein by tissues (white and brown adipose tissue, and skeletal muscles) did not differ significantly for transgenic and non-transgenic mice. The mRNA levers of hormone-sensitive lipase (HSL) and HSL activity in adipose tissue during feeding were higher in LPL transgenic mice than in controls. Results suggest that the overexpression of LPL does not induce obesity by enhancing the hydrolysis of triglycerides in adipose tissue.