Sphingomonas hankyongensis sp. nov. isolated from tap water

• Published in: Archives of microbiology Vol. 198; no. 8; pp. 767 - 771
• Main Authors: Yun, Sung-Sik, Siddiqi, Muhammad Zubair, Lee, Soon-Youl, Kim, Minseok S., Choi, KangDuk, Im, Wan-Taek
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-10-2016; Springer Nature B.V
• Subjects: Bacterial Typing Techniques; Base Composition - genetics; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; DNA, Bacterial - genetics; Drinking water; Drinking Water - microbiology; Ecology; Fatty Acids - analysis; Life Sciences; Microbial Ecology; Microbiology; Original Paper; Phylogenetics; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sphingomonas; Sphingomonas - classification; Sphingomonas - genetics; Sphingomonas - isolation & purification; Taxonomy; Ubiquinone - metabolism; Water Microbiology; South Korea; Tap water; Polyphasic taxonomy; 16S rRNA sequence; Sphingomonas hankyongensis
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-016-1237-1

A Gram reaction-negative, strictly aerobic, non-motile, translucent and rod-shaped bacterium (designated W1-2-4
T
) isolated from tap water was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-4
T
was observed to grow optimally at 25–30 °C and at pH 6.5 on nutrient agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W1-2-4
T
belongs to the genus
Sphingomonas
and is most closely related to the
Sphingomonas fennica
K101
T
(95.3 % similarity). The G+C content of genomic DNA was 67.1 mol%. Chemotaxonomic data [major ubiquinone—Q-10, major polyamine—homospermidine, major fatty acids—summed feature 8 (comprising C
18:1
ω
7
c
/
ω
6
c
), C
16:0
and C
14:0
2OH] supported the affiliation of strain W1-2-4
T
to the genus
Sphingomonas
. Strain W1-2-4
T
could be differentiated genotypically and phenotypically from the recognized species of the genus
Sphingomonas
. The novel isolate therefore represents a novel species, for which the name
Sphingomonas hankyongensis
sp. nov. is proposed, with the type strain W1-2-4
T
(=KACC 18308
T
 = LMG 28595
T
).