Alteration of Striatal Dopamine Levels Under Various Partial Pressure of Oxygen in Pre-convulsive and Convulsive Phases in Freely-Moving rats

• Published in: Neurochemical research Vol. 39; no. 2; pp. 287 - 294
• Main Authors: Lavoute, Cécile, Weiss, Michel, Risso, Jean-Jacques, Rostain, Jean-Claude
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-02-2014; Springer Nature B.V
• Subjects: Anesthesia; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Corpus Striatum - metabolism; Dopamine - metabolism; Electrodes; gamma-Aminobutyric Acid - metabolism; Glutamic Acid - metabolism; Hyperbaric Oxygenation; Male; Neurochemistry; Neurology; Neurosciences; Original Paper; Oxygen - metabolism; Partial Pressure; Rats; Rats, Sprague-Dawley; Seizures - metabolism; Monoamine; Hyperoxic seizures; Basal ganglia; Neurotransmission; Hyperbaric oxygen
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-013-1220-z

The purpose of this study was to investigate the change in the striatal dopamine (DA) level in freely-moving rat exposed to different partial pressure of oxygen (from 1 to 5 ATA). Some works have suggested that DA release by the substantia nigra pars compacta (SNc) neurons in the striatum could be disturbed by hyperbaric oxygen (HBO) exposure, altering therefore the basal ganglia activity. Such changes could result in a change in glutamatergic and GABAergic control of the dopaminergic neurons into the SNc. Such alterations could provide more information about the oxygen-induced seizures observed at 5 ATA in rat. DA-sensitive electrodes were implanted into the striatum under general anesthesia. After 1 week rest, awaked rats were exposed to oxygen–nitrogen mixture at a partial pressure of oxygen of 1, 2, 3, 4 and 5 ATA. DA level was monitored continuously (every 3 min) by in vivo voltammetry before and during HBO exposure. HBO induced a decrease in DA level in relationship to the increase in partial pressure of oxygen from 1 ATA to 4 ATA (−15 % at 1 ATA, −30 % at 2 ATA, −40 % at 3 ATA, −45 % at 4 ATA), without signs of oxygen toxicity. At 5 ATA, DA level strongly decreases (−75 %) before seizure which occurred after 27 min ± 7 HBO exposure. After the epileptic seizure the decrease in DA level disappeared. These changes and the biphasic effect of HBO were discussed in function of HBO action on neurochemical regulations of the nigro striatal pathway.