Blastococcus colisei sp. nov, isolated from an archaeological amphitheatre

• Published in: Antonie van Leeuwenhoek Vol. 110; no. 3; pp. 339 - 346
• Main Authors: Hezbri, Karima, Nouioui, Imen, Rohde, Manfred, Schumann, Peter, Gtari, Maher, Klenk, Hans-Peter, Montero-Calasanz, Maria del Carmen, Ghodhbane-Gtari, Faten
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-03-2017; Springer Nature B.V
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - physiology; Amino acids; Archaeology; Bacteria; Biomedical and Life Sciences; Cell Wall - chemistry; DNA, Bacterial - genetics; Fatty Acids - analysis; Life Sciences; Limestone; Medical Microbiology; Microbiology; Original Paper; Phenotype; Phospholipids - analysis; Phylogeny; Plant Sciences; RNA, Ribosomal, 16S - genetics; Sodium Chloride; Soil Microbiology; Soil Science & Conservation; Species Specificity; Taxonomy; Tunisia; Vitamin K 2 - analysis; Tunisia; Novel species; Polyphasic approach; Geodermatophilaceae
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-016-0804-4

The taxonomic position of an actinobacterial isolate, designated strain BMG 822
T
, isolated from limestone from the Amphitheater of El Jem (Coliseum Thysdrus), Tunisia, was established using a polyphasic approach. Strain BMG 822
T
was found to grow well at 30 °C and pH 6.5–8.0, and to be coral-coloured, Gram-positive, catalase and oxidase negative. Whole cell hydrolysates contained
meso
-diaminopimelic acid as the diagnostic diamino acid, glucose, galactose and ribose. The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, an unidentified glycophospholipid and six unidentified phospholipids. MK-9(H
4
) was found to be the predominant menaquinone, followed by MK-9(H
2
) and MK-9. The major cellular fatty acids were identified as iso-C
16:0
, C
18:1
ω
9c, C
17:1
ω
8c and iso-H-C
16:1
. The G+C content of the DNA (73.2%) is typical of the genus. High degrees of 16S rRNA gene sequence similarity were found with the type strains of the genus
Blastococcus
(97.1–98.3%) followed by the type strains of
Modestobacter
(96.8–97.8%). Based on the above data and the phenotypic differences from the type strains of 
Blastococcus
species, it is proposed that the isolate BMG 822
T
(=DSM 46837
T
=CECT 8823
T
) should be classified as the type strain of a novel species, 
Blastococcus colisei
sp. nov.