Biosynthesis and secretion of fibronectin in the cultured mesenchymal cells of the developing chick Müllerian duct

• Published in: Developmental biology Vol. 139; no. 2; pp. 244 - 249
• Main Authors: Teng, Ching Sung, Huyan, Sile, Ye, Bai Kuan
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01-06-1990; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Separation - methods; Cells, Cultured; Chick Embryo; CHICKS; DESARROLLO EMBRIONARIO; DEVELOPPEMENT EMBRYONNAIRE; Embryology: invertebrates and vertebrates. Teratology; EMBRYONIC DEVELOPMENT; Enzyme-Linked Immunosorbent Assay; Experimental organogenesis; Fibronectins - biosynthesis; Fibronectins - isolation & purification; Fibronectins - metabolism; Fundamental and applied biological sciences. Psychology; Intestinal Mucosa - metabolism; Intestines - cytology; Intestines - embryology; MORFOGENESIS; MORPHOGENESE; MORPHOGENESIS; Mullerian Ducts - cytology; Mullerian Ducts - metabolism; Organogenesis. Physiological fonctions; POLLITO; POUSSIN; SECRECION; SECRETION; Cell culture; Embryonic development; Secretion; Genital system; Mesenchyma; Cell cell interaction; Biosynthesis; Glycoproteins; Fibronectin; Vertebrata; Mullerian duct; Chicken; Aves; Differentiation
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/0012-1606(90)90293-R

We have developed a method to separate and isolate the mesenchymal cells from the epithelial cells in the left Müllerian duct of the developing chick. We then cultured the mesenchymal cells in a serum-free medium. Through an enzyme-linked immunosorbent assay, we detected fibronectin synthesis and release into the medium at stages of Müllerian duct development. Our results demonstrate that the amount of fibronectin secreted by cultured cells gradually decreased in accordance with Müllerian duct differentiation. Similar observations found in the developing embryonic intestine indicate that the highest fibronectin synthesis occurs during early stages of development, when morphogenetic movement and mesenchymal-epithelial interaction are prominent features of embryonic organ differentiation and growth.