Expression of trkC in a mouse osteoblastic cell line and its response to neurotrophin-3

• Published in: Biochemical and biophysical research communications Vol. 203; no. 2; p. 1268
• Main Authors: Nakanishi, T, Ohyama, K, Aoki, C, Kudo, A, Hattori, T, Takahashi, K, Taniguchi, S, Takigawa, M
• Format: Journal Article
• Language: English
• Published: United States 15-09-1994
• Subjects: Animals; Base Sequence; Calcium - metabolism; Cell Differentiation; Cell Division; Cell Line; Gene Expression; Mice; Molecular Sequence Data; Nerve Growth Factors - pharmacology; Neurotrophin 3; Osteoblasts - drug effects; Osteoblasts - metabolism; Polymerase Chain Reaction; Receptor Protein-Tyrosine Kinases - genetics; Receptor, trkC; Receptors, Nerve Growth Factor - genetics; RNA, Messenger - analysis; RNA, Messenger - metabolism
• ISSN: 0006-291X
• DOI: 10.1006/bbrc.1994.2319

In a cultured osteoblastic cell line, MC3T3-E1 derived from newborn mouse calvaria, the mRNA encoding TRKC, which is the receptor molecule of neurotrophin-3 (NT-3), was detected by the polymerase chain reaction (PCR) method. The mRNAs of the normal type and one alternative form (C14) were highly expressed in the exponential growth phase of MC3T3-E1 cells and decreased as the cells reached the differentiation stage. NT-3, but not nerve growth factor (NGF), stimulated the proliferation of MC3T3-E1 cells in a dose-dependent manner. NT-3 also stimulated calcium incorporation through the surface of MC3T3-E1 cells, indicating the association of NT-3 and its receptor on the cell surface.