Chemical Composition of Animal Cells and Their Intracellular Compartments Reconstructed from 3D Mass Spectrometry

Chemical Composition of Animal Cells and Their Intracellular Compartments Reconstructed from 3D Mass Spectrometry

A peek inside: A C60+ sputter ion source integrated into a time‐of‐flight secondary‐ion mass spectrometer was used to dissect animal cells in a layer‐by‐layer fashion and analyze the spatial distribution of endogenous molecules. Repeated cycles of sputter erosion and SIMS analysis provided molecular...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) Vol. 46; no. 28; pp. 5332 - 5335
Main Authors: Breitenstein, Daniel, Rommel, Christina E, Möllers, Rudolf, Wegener, Joachim, Hagenhoff, Birgit
Format: Journal Article
Language:English
Published: Weinheim Wiley-VCH Verlag 01-01-2007
WILEY-VCH Verlag
WILEY‐VCH Verlag
Subjects:
analytical methods
Animals
cell imaging
Cells, Cultured
mass spectrometry
Mass Spectrometry - methods
surface analysis
Online Access:Get full text
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Summary:A peek inside: A C60+ sputter ion source integrated into a time‐of‐flight secondary‐ion mass spectrometer was used to dissect animal cells in a layer‐by‐layer fashion and analyze the spatial distribution of endogenous molecules. Repeated cycles of sputter erosion and SIMS analysis provided molecular information from the inside of the cells (see picture; red: amino acids, green: phospholipids, blue: glass substrate).
Bibliography:http://dx.doi.org/10.1002/anie.200604468
ArticleID:ANIE200604468
istex:317E7B93E8F9BC112292DE41B043C88E068F5529
ark:/67375/WNG-BSJKVMFH-T
German Ministry for Education and Research (BMBF) - No. 0312002A
European Union - No. FP6-513698; No. FP6-005045
This study was financially supported by the German Ministry for Education and Research (BMBF) under grant number 0312002A (Nanobiotechnology) and the European Union under grant numbers FP6-513698 (Toxdrop) and FP6-005045 (Nanobiomaps). The authors acknowledge the help of M. Fartmann for careful revision of the manuscript, S. Grunewald for her expert help with the cell cultures, and both R. Kersting and E. Tallarek for technical advice. We also thank E. Niehuis for helpful discussions and J. Zehnpfenning for providing software support.
This study was financially supported by the German Ministry for Education and Research (BMBF) under grant number 0312002A (Nanobiotechnology) and the European Union under grant numbers FP6‐513698 (Toxdrop) and FP6‐005045 (Nanobiomaps). The authors acknowledge the help of M. Fartmann for careful revision of the manuscript, S. Grunewald for her expert help with the cell cultures, and both R. Kersting and E. Tallarek for technical advice. We also thank E. Niehuis for helpful discussions and J. Zehnpfenning for providing software support.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200604468