Chemical Primer Extension in Seconds

Rapid extention: Catalysis by pyridine accelerates template‐directed chemical primer extension to t1/2<1 min for all four nucleotides (A/C/G/T). For C and G, greater than 99 % conversion is observed after 30 s (see picture). These sequence‐selective reactions open up new avenues for the interroga...

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Bibliographic Details
Published in:	Angewandte Chemie (International ed.) Vol. 47; no. 32; pp. 6065 - 6068
Main Authors:	Röthlingshöfer, Manuel, Kervio, Eric, Lommel, Tanja, Plutowski, Ulrich, Hochgesand, Annette, Richert, Clemens
Format:	Journal Article
Language:	English
Published:	Weinheim Wiley-VCH Verlag 28-07-2008 WILEY-VCH Verlag WILEY‐VCH Verlag
Subjects:	Catalysis DNA DNA Primers Nucleic Acid Amplification Techniques - methods oligonucleotides organocatalysis primer extension Pyridines template synthesis
Online Access:	Get full text
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Summary:	Rapid extention: Catalysis by pyridine accelerates template‐directed chemical primer extension to t1/2<1 min for all four nucleotides (A/C/G/T). For C and G, greater than 99 % conversion is observed after 30 s (see picture). These sequence‐selective reactions open up new avenues for the interrogation of DNA sequences and chemical replication.
Bibliography:	http://dx.doi.org/10.1002/anie.200801260 istex:A4B44497A6C4B098D5D56E641D80D0BC1439A113 This work was supported by the DFG (grant no. RI 1063/1-4 to C.R.). The authors thank Prof. W. Pfleiderer for the gift of an NPPOC precursor. DFG - No. RI 1063/1-4 ark:/67375/WNG-GS4TRPH0-G ArticleID:ANIE200801260 This work was supported by the DFG (grant no. RI 1063/1‐4 to C.R.). The authors thank Prof. W. Pfleiderer for the gift of an NPPOC precursor. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200801260