Effects of Caffeic Acid Phenethyl Ester on Endotoxin-Induced Uveitis in Rats

• Published in: Current eye research Vol. 30; no. 9; pp. 755 - 762
• Main Authors: Yilmaz, Ayça, Yildirim, Özlem, Tamer, Lülüfer, Öz, Özay, Cinel, Leyla, Vatansever, Halil, De irmenci, Ula, Kanik, Arzu, Atik, U ur
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01-09-2005; Taylor & Francis
• Subjects: Animals; Anti-Inflammatory Agents - therapeutic use; anti-inflammatory effects; antioxidants; Antioxidants - therapeutic use; Aqueous Humor - cytology; Aqueous Humor - metabolism; caffeic acid phenethyl ester; Caffeic Acids - therapeutic use; Ciliary Body - metabolism; Ciliary Body - pathology; Disease Models, Animal; endotoxin-induced uveitis; Eye Proteins - metabolism; Injections, Intraperitoneal; Iris - metabolism; Iris - pathology; lipopolysaccharide; Lipopolysaccharides; Male; Peroxidase - metabolism; Phenylethyl Alcohol - analogs & derivatives; Rats; Rats, Wistar; Salmonella typhimurium; Uveitis - chemically induced; Uveitis - drug therapy
• ISSN: 0271-3683; 1460-2202
• DOI: 10.1080/02713680590967962

Purpose: Caffeic acid phenethyl ester (CAPE) has antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, and carcinostatic properties. In this study, the efficacy of CAPE in endotoxin-induced uveitis (EIU) in rats is investigated. Methods: EIU was induced by a footpad injection of lipopolysaccharide (LPS). In the treatment group, 10 μ mol/kg CAPE was injected intraperitoneally immediately after LPS injection. At 24 hr after LPS injection, the number of infiltrating cells, protein concentration, and levels of myeloperoxidase (MPO) in aqueous humor; malondialdehyde (MDA), MPO, and total antioxidant levels in serum were determined. Eyes were enucleated for histopathologic evaluation, and, counting inflammatory cells in iris-ciliary body (ICB), the efficacy of treatment was determined. Results: CAPE significantly suppressed LPS-induced increase in the number of inflammatory cells (p = 0.0001), protein concentration (p = 0.0001), and MPO levels (p = 0.0001) in aqueous humor as well as MDA (p = 0.001) and MPO (p = 0.0001) levels in serum. Histopathologic evaluation of ICB showed significant reduction in the inflammatory cell counts in the treatment group (p = 0.0001). Conclusions: CAPE was found efficient in suppressing inflammation and ocular tissue damage induced by LPS in rats.