Genotypes of Leptospira spp. strains isolated from dogs in Buenos Aires, Argentina

• Published in: Revista argentina de microbiología Vol. 46; no. 3; pp. 201 - 204
• Main Authors: Loffler, Sylvia Grune, Passaro, Diego, Samartino, Luis, Soncini, Analía, Romero, Graciela, Brihuega, Bibiana
• Format: Journal Article
• Language: English
• Published: Argentina Elsevier España, S.L.U 01-07-2014
• Subjects: Animals; Argentina - epidemiology; Caracterización molecular; Disease Reservoirs; Dog Diseases - epidemiology; Dog Diseases - microbiology; Dogs - microbiology; Endemic Diseases - veterinary; Genotyping Techniques; Leptospira - classification; Leptospira - genetics; Leptospira - isolation & purification; Leptospira interrogans - classification; Leptospira interrogans - genetics; Leptospira interrogans - isolation & purification; Leptospira spp; Leptospirosis - epidemiology; Leptospirosis - microbiology; Leptospirosis - veterinary; Minisatellite Repeats; MLVA; Molecular characterization; Phylogeny; Species Specificity; Urban Health; Argentina; Molecular characterization; Caracterización molecular; Leptospira spp; MLVA
• ISSN: 0325-7541
• DOI: 10.1016/S0325-7541(14)70073-2

Leptospirosis is an infectious disease of wide global distribution, which is endemic in Argentina. The objective of this study was to obtain the genetic profiles of Leptospira spp. strains isolated from clinical cases of dogs in the province of Buenos Aires by the multiple-locus variable-number tandem repeat analysis (MLVA). Eight isolated canine strains were genotyped by MLVA, obtaining the identical profile of Leptospira interrogans serovar Canicola Hond Utrecht IV in the strains named Dogy and Mayo. The strains named Bel, Sarmiento, La Plata 4581 and La Plata 5478 were identical to the profile of the genotype of L. interrogans serovar Portlandvere MY 1039.The strain named Avellaneda was identical to the genotype profile of L. interrogans serovar Icterohaemorrhagiae RGA and the strain named SB had the same profile as the L. interrogans serovar Pomona Baires genotype and was similar to the profile of serovar Pomona Pomona genotype. It would be useful to include a larger number of isolates from different dog populations in various provinces of Argentina and to characterize the genetic profiles of the strains circulating in the country. The information obtained will be useful for the control of leptospirosis in the dog population.
La leptospirosis es una enfermedad infecciosa de amplia distribución global; endémica en Argentina. El objetivo de este estudio fue obtener los perfiles genéticos de las cepas de Leptospira spp. aisladas de casos clínicos de perros provenientes de la provincia de Buenos Aires, empleando el análisis de repeticiones en tándem de número variable en múltiples locus [multiple-locus variable-number tandem repeats analysis (MLVA)]. Fueron genotipificadas por MLVA ocho cepas aisladas de perros. Se obtuvo un perfil idéntico al de Leptospira interrogans serovar Canicola Hond Utrecht IV en las cepas denominadas Dogy y Mayo. Las cepas denominadas Bel, Sarmiento, La Plata 4581 y La Plata 5478 mostraron un perfil idéntico al genotipo de L. interrogans serovar Portlandvere MY 1039. La cepa denominada Avellaneda presentó un perfil idéntico al genotipo L. interrogans serovar Icterohaemorrhagiae RGA, y la cepa denominada SB mostró un perfil idéntico al genotipo de L. interrogans serovar Pomona Baires y similar al serovar Pomona Pomona. Sería de gran utilidad incluir un mayor número de cepas provenientes de distintas poblaciones caninas de diversas provincias de Argentina a fin de conocer los perfiles de las cepas circulantes en el país. La información así obtenida contribuirá al control de la leptospirosis en la población canina.