Highly diverse-Low abundance methanogenic communities in hypersaline microbial mats of Guerrero Negro B.C.S., assessed through microcosm experiments

• Published in: PloS one Vol. 19; no. 10; p. e0303004
• Main Authors: Ramírez-Arenas, Patricia J, Latisnere-Barragán, Hever, García-Maldonado, José Q, López-Cortés, Alejandro
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04-10-2024
• Subjects: Abundance; Archaea; Archaea - classification; Archaea - genetics; Archaea - metabolism; Biodiversity; Carbon dioxide; Carbon Dioxide - metabolism; Chromatography; Closed ecological systems; Environmental aspects; Environmental studies; Experiments; Gene sequencing; Growth; Hydrogen; Hydrogen - metabolism; Identification and classification; Mesocosms; Methane - metabolism; Methanobacteriaceae; Methanogenic archaea; Methanogenic bacteria; Methylamines - metabolism; Mexico; Microbial mats; Microbiota; Microcosms; Microorganisms; Next-generation sequencing; Phylogeny; Relative abundance; Salinity; Substrates; Taxonomy; Trimethylamine; Mexico; Baja California Mexico; United States > US; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0303004

Methanogenic communities of hypersaline microbial mats of Guerrero Negro, Baja California Sur, Mexico, have been recognized to be dominated by methylotrophic methanogens. However, recent studies of environmental samples have evidenced the presence of hydrogenotrophic and methyl-reducing methanogenic members, although at low relative abundances. Physical and geochemical conditions that stimulate the development of these groups in hypersaline environments, remains elusive. Thus, in this study the taxonomic diversity of methanogenic archaea of two sites of Exportadora de Sal S.A was assessed by mcrA gene high throughput sequencing from microcosm experiments with different substrates (both competitive and non-competitive). Results confirmed the dominance of the order Methanosarcinales in all treatments, but an increase in the abundance of Methanomassiliiccocales was also observed, mainly in the treatment without substrate addition. Moreover, incubations supplemented with hydrogen and carbon dioxide, as well as the mixture of hydrogen, carbon dioxide and trimethylamine, managed to stimulate the richness and abundance of other than Methanosarcinales methanogenic archaea. Several OTUs that were not assigned to known methanogens resulted phylogenetically distributed into at least nine orders. Environmental samples revealed a wide diversity of methanogenic archaea of low relative abundance that had not been previously reported for this environment, suggesting that the importance and diversity of methanogens in hypersaline ecosystems may have been overlooked. This work also provided insights into how different taxonomic groups responded to the evaluated incubation conditions.