Determination of trace mercury in compost extract by inhibition based glucose oxidase biosensor

• Published in: Transactions of Nonferrous Metals Society of China Vol. 19; no. 1; pp. 235 - 240
• Main Author: 刘剑潇 徐祥民 汤琳 曾光明
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-02-2009; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, China%College of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, China; College of Environmental Science and Engineering, Hunan University, Changsha 410082, China%College of Environmental Science and Engineering, Hunan University, Changsha 410082, China
• Subjects: biosensor; compost; glucose oxidase; inhibition; mercury; 性能参数; 有色金属; 金属学; inhibition; biosensor; mercury; compost; glucose oxidase
• ISSN: 1003-6326
• DOI: 10.1016/S1003-6326(08)60258-7

A novel inhibition based biosensor of glucose oxidase（GOx） for environmental mercury detection was developed. An electropolymerized aniline membrane was prepared on a platinum electrode containing ferrocene as electron transfer mediator, on which GOx was cross-linked by glutaraldehyde. The response of the sensor was based on the current reduction in the electrochemical system by inhibition of mercury against GOx electrode. The detection limit of the inhibition-based sensor for mercury is 0.49 μg/L, and the linear response ranges are 0.49 783.21 gg/L and 783.21 μg/L-25.55 mg/L. The GOx membrane can be completely reactivated after inhibition, and remains 70% of the activity in more than one month. The sensor was used for mercury determination in compost extract with good results.