Effects of Navelbine and Docetaxel on Gene Expression in Human Lung Cancer Cell Lines

• Published in: Cell biochemistry and biophysics Vol. 61; no. 3; pp. 665 - 671
• Main Authors: Shi, Guangyue, Cai, Li, Jiang, Wei, Sui, Guangjie
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01-12-2011; Springer Nature B.V
• Subjects: Antineoplastic Agents - pharmacology; Antitumor agents; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biotechnology; Cell Biology; Cell Line, Tumor; Dissolved organic carbon; Enzymes; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Humans; Life Sciences; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Non-small cell lung carcinoma; Original Paper; Pharmacology/Toxicology; Signal transduction; Taxoids - pharmacology; Transcription factors; Transcriptome - drug effects; Tumor cell lines; Vinblastine - analogs & derivatives; Vinblastine - pharmacology; Lung cancer; Docetaxel; MTT; Gene expression; Navelbine; cDNA Macroarray; Antitumor drugs
• ISSN: 1085-9195; 1559-0283
• DOI: 10.1007/s12013-011-9252-y

In this study, we identified the genes in small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) cell lines sensitive to anticancer drugs. For this purpose, sensitivity of four SCLC and six NSCLC cell lines to navelbine (NVB) and docetaxel (DOC) was evaluated using MTT assay. Expression of 1,`291 drug sensitivity-related genes in these cells was measured by cDNA macroarrays, and their relationship was analyzed. Overall, there were 56 (
r
 ≥ ± 0.4) genes sensitive to NVB and DOC. Regarding NVB, 36 genes were sensitive to NVB, 20 co-expressed genes between SCLC+NSCLC and NSCLC sets; 27 expressed and seven specially expressed genes in SCLC+NSCLC set; and 29 expressed and nine specially expressed genes in NSCLC set. Concerning DOC, 50 genes were sensitive to DOC, 12 co-expressed genes between SCLC+NSCLC and NSCLC sets; 24 expressed and 12 specially expressed genes in SCLC+NSCLC set; and 38 expressed and 26 specially expressed genes in NSCLC set. The genes sensitive to NVB and DOC in these cell lines were categorized into signal transduction molecules, cell factors, transcription factors and metabolism-related enzymes and their inhibitors. In conclusion, there were gene expression differences between SCLC and NSCLC cell lines related to NVB and DOC; nevertheless, these genes also shared functional similarities.