Loss of cytochrome c Fe(III)/Fe(II) redox couple in ionic liquids

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Vol. 553; pp. 187 - 190
• Main Authors: Compton, David L., Laszlo, Joseph A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30-08-2003; Elsevier Science
• Subjects: Animal cells; Biological and medical sciences; Cell cultures. Hybridization. Fusion; Cyclic voltammetry; Cytochrome c; Fundamental and applied biological sciences. Psychology; Ionic liquids; Molecular and cellular biology; Self assembled monolayer; Cyclic voltammetry; Cytochrome c; Ionic liquids; Self assembled monolayer; Self-assembled layer; Solvent effect; Self assembly; Monolayer; Gold; Hemoprotein; Thiol; Bioelectrochemistry; Electrochemical activity; Hexafluophosphates; Transition metal; Experimental study; Ionic liquid; Electrodes; Redox couple; Adsorbed state; Modified material
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/S0022-0728(03)00324-3

The electrochemical behavior of cytochrome
c (cyt-
c) adsorbed to gold electrodes through mercaptoalkanoic acid/mercaptoalcohol monolayers was investigated in the 1-butyl-3-methylimidazolium salts of bis(trifluoromethylsulfonyl)imide and hexafluorophosphate. Cyt-
c does not retain its Fe(III)/Fe(II) redox activity in dry ionic liquids (ILs); however, the redox signal of the modified electrodes can be reconstituted upon subsequent treatment with aqueous buffer. Water saturated ILs, 1.4 wt.%, failed to support the redox activity of the adsorbed cyt-
c; therefore, the ILs’ deleterious effects on the redox behavior of the cyt-
c modified electrodes cannot be attributed solely to the stripping of essential water from the enzyme's polypeptide lattice.