Alleged reaction in gel-immunodiffusion of an IgM monoclonal antibody with alfalfa mosaic virus and cucumber mosaic virus is an artefact

• Published in: Journal of general virology Vol. 75; no. 2; pp. 463 - 465
• Main Authors: Hajimorad, M. R, Dietzgen, R. G, Wahyuni, W. S, Francki, R. I. B
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-02-1994; Society for General Microbiology
• Subjects: Alfalfa mosaic virus - immunology; Antibodies, Monoclonal - immunology; Biological and medical sciences; Cross Reactions; Cucumovirus - immunology; Fundamental and applied biological sciences. Psychology; Immunodiffusion; Immunoglobulin M - immunology; Microbiology; Techniques used in virology; Virology; Virus; Specificity; Immunodiffusion; Cucumber mosaic virus; Cross reaction; Alfalfa mosaic virus; Cucumovirus; Artefact; Monoclonal antibody; IgM
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-75-2-463

Department of Crop Protection, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia 5064, Australia
A previously reported spurious serological cross-reaction between alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV), which had been defined by the reaction in gel-immunodiffusion tests of a single IgM monoclonal antibody (MAb), MAb 8, was no longer detected in the presence of 0.1 M -NaCl. The non-specific reactivity of this IgM was also confirmed in Western blotting assays. When skimmed milk was used as a blocking agent and as a diluent of antibodies, MAb 8 failed to recognize AMV and CMV coat proteins. Hence, it is concluded that the alleged cross-reaction between AMV and CMV is due to non-specific binding of MAb 8 and that there is no evidence for a serological relationship between these two viruses.
Present address: Agricultural Research Organization, Plant Pests and Diseases Research Institute, P.O. Box 1454, Tehran 19395, Iran.
Present address: Queensland Department of Primary Industries, Plant Protection Division and Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, University of Queensland, Queensland 4072, Australia.
Present address: Faculty of Agriculture, University of Jember, 3/23 Kalimantan Street, Jember 68121, East Java, Indonesia.
|| Deceased, 14 November 1990.
Received 20 July 1993;
accepted 21 September 1993.