The point spread function of optical microscopes imaging through stratified media

We propose a model for imaging point objects through a dielectric interface or stratified media. The model is applicable to conventional and confocal fluorescence microscopy, with single- or multiphoton excitation. An analytical solution is obtained in the form of readily computable functions. When...

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Bibliographic Details
Published in:	Optics express Vol. 11; no. 22; pp. 2964 - 2969
Main Authors:	Haeberlé, O, Ammar, M, Furukawa, H, Tenjimbayashi, K, Török, P
Format:	Journal Article
Language:	English
Published:	United States Optical Society of America - OSA Publishing 03-11-2003
Subjects:	Engineering Sciences Optics Photonic
Online Access:	Get full text
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Summary:	We propose a model for imaging point objects through a dielectric interface or stratified media. The model is applicable to conventional and confocal fluorescence microscopy, with single- or multiphoton excitation. An analytical solution is obtained in the form of readily computable functions. When large mismatches occur in the refractive indices of the media of the objective lens and specimen the illumination and detection point spread functions differ significantly, showing that currently used imaging models may fail to correctly predict imaging properties of optical microscopes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1094-4087 1094-4087
DOI:	10.1364/OE.11.002964