Proton-irradiated breast cells: molecular points of view

• Published in: Journal of radiation research Vol. 60; no. 4; pp. 451 - 465
• Main Authors: Bravatà, Valentina, Cammarata, Francesco P, Minafra, Luigi, Pisciotta, Pietro, Scazzone, Concetta, Manti, Lorenzo, Savoca, Gaetano, Petringa, Giada, Cirrone, Giuseppe A P, Cuttone, Giacomo, Gilardi, Maria C, Forte, Giusi I, Russo, Giorgio
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01-07-2019
• Subjects: Breast - radiation effects; Breast Neoplasms - radiotherapy; Cell Line, Tumor; DNA, Complementary - radiation effects; Dose-Response Relationship, Radiation; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - radiation effects; Humans; Inflammation; MCF-7 Cells; Oligonucleotide Array Sequence Analysis; Phenotype; Proton Therapy; Protons; Radiation Tolerance - genetics; Radiotherapy; Regular Paper; breast cancer; cDNA microarray; proton therapy; gene signature; radiation
• ISSN: 0449-3060; 1349-9157
• DOI: 10.1093/jrr/rrz032

Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and describe the production of immunological molecules and gene expression profiles induced by proton irradiation. We performed Luminex assay and cDNA microarray analyses to study the biological processes activated following irradiation with proton beams, both in the non-tumorigenic MCF10A cell line and in two tumorigenic BC cell lines, MCF7 and MDA-MB-231. The immunological signatures were dose dependent in MCF10A and MCF7 cell lines, whereas MDA-MB-231 cells show a strong pro-inflammatory profile regardless of the dose delivered. Clonogenic assay revealed different surviving fractions according to the breast cell lines analyzed. We found the involvement of genes related to cell response to proton irradiation and reported specific cell line- and dose-dependent gene signatures, able to drive cell fate after radiation exposure. Our data could represent a useful tool to better understand the molecular mechanisms elicited by proton irradiation and to predict treatment outcome.