A novel urea biosensor based on zirconia

• Published in: Thin solid films Vol. 519; no. 3; pp. 1187 - 1191
• Main Authors: Sumana, G., Das, Maumita, Srivastava, Saurabh, Malhotra, B.D.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 30-11-2010; Elsevier
• Subjects: Biosensor; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Electrochemical deposition; Electrodeposition, electroplating; Exact sciences and technology; Glutamate dehydrogenase; Keto glutamate; Materials science; Methods of deposition of films and coatings; film growth and epitaxy; Nanoscale materials and structures: fabrication and characterization; Nanostructured metal oxide; Other topics in nanoscale materials and structures; Physics; Structure and morphology; thickness; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Thin film structure and morphology; Urea; Urease; Zirconia; Zirconia; Electrochemical deposition; Glutamate dehydrogenase; Urea; Nanostructured metal oxide; Biosensor; Keto glutamate; Urease; Enzymes; Scanning electron microscopy; Fourier transform spectroscopy; Electrochemical method; Nanostructures; Thin films; Zirconium oxide; Biocompatibility; Electrodeposition; Biosensors
• ISSN: 0040-6090; 1879-2731
• DOI: 10.1016/j.tsf.2010.08.067

Electrochemically deposited biocompatible zirconia (ZrO
2) film on gold coated glass electrodes has been utilized for the fabrication of urea biosensor. The prepared ZrO
2 films and bioelectrodes have been characterized using Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) and electrochemical techniques, respectively. The urea biosensor, fabricated by immobilizing mixed enzyme [urease (Urs) and glutamate dehydrogenase (GLDH)] on this nanobiomaterial, shows linearity up to 40
mg
dL
−
1
of analyte (urea) and sensitivity of 0.071
μA/(mM
cm
−
2
) with stability up to 4
months when stored at 4
°C. The low value of Michaelis–Menten constant (
K
m
) estimated using Hans plot as 0.5
mM indicates enhancement in the affinity and/or activity of enzyme attached to this nanostructured biocompatible matrix.