Tetracarboxy-phthalocyanines: From excited state dynamics to photodynamic inactivation against Bovine herpesvirus type 1

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 175; pp. 1 - 8
• Main Authors: Cocca, Leandro H.Z., Oliveira, Taise M.A., Gotardo, Fernando, Teles, Amanda V., Menegatti, Ricardo, Siqueira, Jonathas P., Mendonça, Cleber R., Bataus, Luiz A.M., Ribeiro, Anderson O., Souza, Thalita F.M., Souza, Guilherme R.L., Gonçalves, Pablo J., De Boni, Leonardo
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01-10-2017; Elsevier BV
• Subjects: Aluminum; Animal diseases; Animal health; Animals; Cattle; Data processing; Deactivation; Excitation; Fluorescence; Herpesvirus 1, Bovine - physiology; Inactivation; Indoles - chemistry; Indoles - pharmacology; Irradiation; Laboratory equipment; Light; Microorganisms; Optical limiters; Optical properties; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacology; Photodynamic therapy; Photosensitizing Agents - chemistry; Photosensitizing Agents - pharmacology; Quantum Theory; Singlet Oxygen - chemistry; Singlet Oxygen - metabolism; Triplet state; Veterinary medicine; Viability; Virus Inactivation - drug effects; Virus Inactivation - radiation effects; Viruses; White light; Zinc
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2017.08.019

Herein we present the excited state dynamic of zinc and aluminum tetracarboxy-phthalocyanines (ZnPc and AlPc) and its application in the photodynamic inactivation (PDI) of Bovine herpesvirus type 1 (BoHV-1) in vitro. The excited state dynamic provides valuable data to describe the excited state properties of potential optical limiters and/or photosensitizers (PSs), such as: the excited state cross-sections, fluorescence lifetime and triplet state quantum yield. The excited state characterization was performed using three different Z-scan techniques: Single Pulse, White Light Continuum and Pulse Train. Considering the photodynamic inactivation of BoHV-1, an initial viral suspension containing 105.75TCID50/mL was incubated with the PSs for 1h at 37°C under agitation and protected from light. The samples were placed in microtiter plates and irradiated (180mW/cm2). During irradiation, a sample was taken every 15min and the viability of the virus was evaluated. The results show that both phthalocyanines were efficient against viruses. However, a higher photodynamic efficiency was observed by ZnPc, which can be attributed to its higher triplet and singlet quantum yields. The results presented here are important for animal health (treatment of BoHV-1) and also open up a field of studies to use AlPc and ZnPc as potential agents against a wide range of microorganisms of veterinary interest.
•Excited states dynamic of zinc and aluminum tetracarboxy-phthalocyanines•Photodynamic inactivation of Bovine herpesvirus type 1 (BoHV-1) in vitro•The results presented are important for animal health (treatment of BoHV-1).•This work opens up a field of studies against microorganism of veterinary interest.