Phenol-containing saline solution as a diluent for adenosine 5'-monophosphate in bronchial challenge testing

• Published in: Chest Vol. 127; no. 1; p. 125
• Main Authors: Prieto, Luis, Badiola, Carlos, Cortijo, Julio, Pérez-Francés, Carmen, Gutiérrez, Valentina, Lanuza, Amparo
• Format: Journal Article
• Language: English
• Published: United States 01-01-2005
• Subjects: Adenosine Monophosphate - administration & dosage; Adenosine Monophosphate - chemistry; Administration, Inhalation; Adult; Asthma - diagnosis; Bronchial Provocation Tests; Chromatography, High Pressure Liquid; Female; Humans; Male; Middle Aged; Phenols - analysis; Sodium Chloride - chemistry
• ISSN: 0012-3692
• DOI: 10.1378/chest.127.1.125

To investigate the effect of dissolving adenosine 5'-monophosphate (AMP) with phenol-containing saline solution on the stability and the bronchoconstrictive properties of this indirect agonist.
Eleven subjects with asthma well controlled with short-acting inhaled beta2-agonists as required or with inhaled corticosteroids were studied. Bronchial challenge tests with AMP dissolved with either normal saline solution or saline solution containing 0.4% phenol were performed on separate days. Furthermore, to assess the potential influence of the phenol-containing saline solution on the stability of the bronchoconstrictor agent, AMP solutions in concentrations of 40 microg/mL and 400 microg/mL were prepared in saline solution and phenol-containing saline solution and, after 30 min, the AMP levels were determined by high-performance liquid chromatography (HPLC) assay.
The geometric mean AMP provocative concentration causing a 20% fall in FEV1 (PC20) was 13.49 mg/mL (95% confidence interval [CI], 6.76 to 26.91) for the saline solution method, and AMP PC20 for the saline solution with phenol method was 8.91 mg/mL (95% CI, 3.39 to 23.44) [p = 0.18]. No significant differences were found between the concentrations of AMP made in saline solution compared to those made in phenol-containing saline solution measured by HPLC.
These observations indicate that normal saline solution with or without phenol can be used as the diluent for AMP. However, since a potential risk with AMP of industrial sources is the bacterial contamination, adding a preservative such as phenol to a saline solution diluent might be recommended.