Measles virus-specific T4+ human cytotoxic T cell clones are restricted by class II HLA antigens

• Published in: The Journal of immunology (1950) Vol. 133; no. 2; pp. 754 - 757
• Main Authors: Jacobson, S, Richert, JR, Biddison, WE, Satinsky, A, Hartzman, RJ, McFarland, HF
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01-08-1984; American Association of Immunologists
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Biological and medical sciences; Clone Cells - immunology; Cytotoxicity, Immunologic; Epitopes - genetics; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Histocompatibility Antigens Class II - genetics; HLA-DP Antigens; HLA-DR Antigens; Humans; Immunobiology; Measles virus - immunology; Multiple Sclerosis - immunology; Organs and cells involved in the immune response; Phenotype; T-Lymphocytes, Cytotoxic - immunology; Human; Cell cloning; HLA-System; Multiple sclerosis; Histocompatibility system; Immune response; Cytotoxicity; Cellular immunity; Monoclonal antibody; Paramyxoviridae; Infection; Antigen; Virus; Specificity; Viral disease; Measles; T-Lymphocyte; Morbillivirus
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.133.2.754

We have generated measles virus-specific T cell clones from a patient with multiple sclerosis who has been described previously as a strong responder to measles virus. T cell clones were screened on the basis of their capacity to proliferate to measles virus. The cell surface phenotype of each clone was OKT3+, OKT4+, OKT8-. The majority of these clones (11 of 14) were cytotoxic for their autologous measles virus-infected lymphoblastoid B cell target. This cytotoxicity was specific for measles virus inasmuch as these T cell clones could not lyse influenza or mumps virus-infected B cell targets. By using a panel of HLA-defined measles virus-infected B cell lines, these clones were shown to recognize measles virus in the context of HLA class II determinants. A monoclonal antibody that recognizes HLA class II monomorphic determinants (L243), but not a monoclonal antibody that recognizes HLA class I antigens (W6/32), inhibited the lysis of the autologous measles virus-infected B cell target by these T cell clones. These results demonstrate that these cytotoxic T cell clones specific for measles virus are HLA class II restricted.