Taenia saginata metacestode antigenic fractions obtained by ion-exchange chromatography: Potential source of immunodominant markers applicable in the immunodiagnosis of human neurocysticercosis

• Published in: Diagnostic microbiology and infectious disease Vol. 76; no. 1; pp. 36 - 41
• Main Authors: da Silva Nunes, Daniela, Gonzaga, Henrique Tomaz, da Silva Ribeiro, Vanessa, da Cunha-Júnior, Jair Pereira, Costa-Cruz, Julia Maria
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-05-2013
• Subjects: Animals; Antibodies, Helminth - blood; Antigens, Helminth - blood; Area Under Curve; Biomarkers; Chromatography, Ion Exchange - methods; Diagnosis; Enzyme-Linked Immunosorbent Assay; Humans; Immunoblotting; Immunologic Tests - methods; Infectious Disease; Internal Medicine; Ion exchange; Neurocysticercosis; Neurocysticercosis - diagnosis; Neurocysticercosis - immunology; Neurocysticercosis - parasitology; Sensitivity and Specificity; Sepharose - chemistry; Taenia saginata - immunology; Taenia saginata - isolation & purification; Taenia saginata metacestodes; Neurocysticercosis; Diagnosis; Ion exchange; Taenia saginata metacestodes
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2013.01.020

Abstract The aim of this study was to fractionate and partially characterize fractions obtained from the total saline extract (SE) of Taenia saginata metacestodes after ion-exchange procedure in carboxymethyl sepharose (CM) and diethylaminoethyl sepharose (DEAE) resins, as a source of antigenic markers applicable in the immunodiagnosis of neurocysticercosis (NCC). For IgG detection by enzyme-linked immunosorbent assay (ELISA) and immunoblotting, 140 serum samples were analyzed: 45 from patients with NCC (G1), 50 from patients with other parasitic infections (G2), and 45 from healthy individuals. Sensitivity (Se), specificity (Sp), area under curve (AUC), and likelihood ratios (LR) were calculated. CM S2 and DEAE S2 fractions provided high diagnostic values (Se 88.8% and 93.4%; Sp 93.7% and 92.6%; AUC 0.965 and 0.987; LR+ 14.07 and 12.67; LR− 0.11 and 0.07, respectively). In conclusion, CM S2 and DEAE S2 fractions are important sources of specific peptides, with high efficiency to diagnose NCC.