use of PCR melting profile for typing of Pseudomonas syringae isolates from stone fruit trees

• Published in: European journal of plant pathology Vol. 126; no. 4; pp. 437 - 443
• Main Authors: Kałużna, Monika, Puławska, Joanna, Sobiczewski, Piotr
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01-04-2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Agriculture; Bacteria; Bacterial plant pathogens; Biological and medical sciences; Biomedical and Life Sciences; Coronatine; Ecology; Fruit trees; Fruits; Fundamental and applied biological sciences. Psychology; Genetic diversity; Life Sciences; Melting; Phytopathology. Animal pests. Plant and forest protection; Plant diseases; Plant Pathology; Plant Sciences; Prunus; Toxins; Trees; Pathogenicity; Toxins; PCR MP; pv; Bacterial canker; Pseudomonadales; Plant pathology; Typing; Plant pathogen; Pseudomonas syringae pv. morsprunorum; Polymerase chain reaction; Toxin; Plant; Pseudomonas syringae; Bacteria; Pseudomonadaceae; Isolate; Pseudomonas syringae pv. syringae
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-009-9553-9

Of thirty fluorescent Pseudomonas isolates originating from symptomatic tissues of sweet (Prunus avium) and sour cherry (Prunus cerasus), plum (Prunus domestica), peach (Prunus persica) and apricot (Prunus armeniaca), 23 were identified as P. syringae using LOPAT tests. Further characterization of those isolates by GATTa and L-lactate utilization tests showed that 10 of them belonged to race 1, six to race 2 of P. syringae pv. morsprunorum (Psm) and six other isolates were identified as pathovar syringae (Pss). One isolate (791) was determined as atypical. Phenotypic determination and genetic analysis of studied isolates for toxin production revealed that isolates of Pss produced syringomycin, 3 Psm race 1 produced coronatine and 6 Psm race 2 produced yersiniabactin. Genetic diversity of all isolates was evaluated with the PCR melting profile (PCR MP) method. A dendrogram constructed with PCR MP patterns showed positive correlation with phenotypically distinguished pathovars. Isolates of Psm races 1 and 2 formed distinct, tight clusters, whereas Pss isolates were more heterogeneous. Isolate 791 was placed within Pss isolates. Bacteria identified as Pss caused more severe symptoms on immature cherry fruits compared to Psm, which corresponded to determined pathovars and races.