Effects of Ni2+ Ions on Cell Viability and NO Production of Murine Peritoneal Exudate Cells (Macrophages) with and without Lipopolysaccharide Stimulation

• Published in: Dental Materials Journal Vol. 24; no. 3; pp. 304 - 310
• Main Authors: TAIRA, Masayuki, SASAKI, Minoru, YAMAURA-TANAKA, Chiharu, SAITOH, Setsuo, KIMURA, Shigenobu, ARAKI, Yoshima
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Society for Dental Materials and Devices 01-09-2005
• Subjects: Actins - analysis; Animals; Cell Survival - drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Free Radical Scavengers - metabolism; Interleukin-1 - analysis; Interleukin-6 - analysis; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - toxicity; LPS; Macrophages, Peritoneal - drug effects; Mice; Nickel - administration & dosage; Nickel - toxicity; Nickel ions; Nitric Oxide - analysis; Nitric Oxide - biosynthesis; Nitric Oxide Synthase - analysis; Nitric Oxide Synthase - drug effects; Peritoneal exudate cells; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Tumor Necrosis Factor-alpha - analysis; Tumor Necrosis Factor-alpha - drug effects
• ISSN: 0287-4547; 1881-1361
• DOI: 10.4012/dmj.24.304

The purpose of this study was to clarify the cytotoxicity of Ni2+ ions against murine peritoneal exudate cells (PEC) (macrophages). First, we examined the cell viability of PEC with and without lipopolysaccharide (LPS) stimulation in culture media containing Ni2+ ions up to 1000μmol/L. Results showed that the cytotoxicity of Ni2+ ions against PEC was dosedependent and accelerated by LPS stimulation, especially in media with Ni2+ ions exceeding 100μmol/L. Second, we measured the production of nitric oxide (NO) from PEC and found that LPS caused the PEC to produce abundant NO. However, high dose of Ni2+ ions at concentration more than 200μmol/L hindered and inhibited NO production. These results pointed out that the cytotoxicity of Ni2+ ions against macrophages depended on both the Ni2+ ion concentration and the presence of bacteria with LPS. Further, NO —a killer of bacteria— was lost when LPS-stimulated macrophages were exposed to high dose of Ni2+ ions.