A simple floral fragrance and unusual osmophore structure in Cyclopogon elatus (Orchidaceae)

• Published in: Plant biology (Stuttgart, Germany) Vol. 11; no. 4; pp. 506 - 514
• Main Authors: Wiemer, A. P., Moré, M., Benitez-Vieyra, S., Cocucci, A. A., Raguso, R. A., Sérsic, A. N.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-07-2009
• Subjects: 7-triene; 8-dimethylnona-1; DMNT; floral scent; Flowers - physiology; Flowers - ultrastructure; GC-MS; Halictidae; Microscopy, Electron, Scanning; Odorants; Orchidaceae - physiology; Orchidaceae - ultrastructure; osmophore; pollinator attraction; trans-4
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1111/j.1438-8677.2008.00140.x

We studied gland morphology, anatomy and the chemical composition of the floral fragrance in the sweat bee-pollinated orchid Cyclopogon elatus. This is apparently the first such analysis for any Cyclopogon species, and one of very few studies in which both odour and osmophore are characterised in a nectar-rewarding orchid. Structures responsible for floral scent production were localised with neutral red staining and histochemical assays for lipids and starch. Their morphology and anatomy were studied with scanning electron microscopy and light microscopy thin sections, respectively. Fragrance samples were collected using SPME fibres and analysed with GC-MS. Anatomical evidence suggests that two parallel oval-shaped patches of unicellular trichomes on the abaxial surface of the labellum are osmophores. These are rich in stored lipids, while the parenchyma surrounding the vascular bundles contains starch. Only freshly opened flowers produced odours, while buds and withered flowers lacked scent. The chemical composition of the odour was dominated (>99.8%) by a single compound, trans-4,8-dimethyl-nona-1,3,7-triene (DMNT). Gland anatomy and position on the outside of the perianth are unusual for scent glands in general. The presence of DMNT, a nearly ubiquitous compound in herbivore-induced vegetative emissions and one of the major floral volatiles of Yucca, is not surprising in view of hypotheses on the evolutionary origin of flower scents, suggesting that wound volatiles are utilised as kairomonal attractants by florivores whose activities result in pollination.