Actinoplanes tropicalis sp. nov. and Actinoplanes cibodasensis sp. nov., isolated from leaf litter
Two actinomycete strains, designated LIPI11-2-Ac034 and LIPI11-2-Ac042 , were isolated from leaf litter collected from Cibodas Botanical Garden, West Java, Indonesia. Phylogenetic analysis based on 16S rRNA gene sequences suggested that both isolates belong to the genus . These isolates were closely...
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| Published in: | International journal of systematic and evolutionary microbiology Vol. 65; no. 11; pp. 3824 - 3829 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
01-11-2015
|
| Online Access: | Get full text |
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| Summary: | Two actinomycete strains, designated LIPI11-2-Ac034
and LIPI11-2-Ac042
, were isolated from leaf litter collected from Cibodas Botanical Garden, West Java, Indonesia. Phylogenetic analysis based on 16S rRNA gene sequences suggested that both isolates belong to the genus
. These isolates were closely related to
and
with similarity values of 98.2 % and 97.7 % respectively, for strain LIPI11-2-Ac034
, and 99.0 % and 97.4-97.7 % respectively for strain LIPI11-2-Ac042
. Both isolates grew well on ISP 7 medium with brown soluble pigment production. Spores were motile and sporangia were irregular. The isolates contained
-diaminopimelic acid in cell-wall hydrolysates, and mannose, glucose and galactose in whole-cell hydrolysates. The predominant menaquinone of strain LIPI11-2-Ac034
was MK-9(H4) while that of strain LIPI11-2-Ac042
was MK-9(H6). The major cellular fatty acids were iso-C
, iso-C
and anteiso-C
for strain LIPI11-2-Ac034
, and iso-C
, anteiso-C
, iso-C
and anteiso-C
for strain LIPI11-2-Ac042
. Phosphatidylethanolamine was detected as the diagnostic polar lipid. The DNA G+C contents of strains LIPI11-2-Ac034
and LIPI11-2-Ac042
were 71.5 and 70.7 mol%, respectively. Based on the differential phenotypic characteristics and the results of DNA-DNA hybridization and phylogenetic analysis, it is proposed that strains LIPI11-2-Ac034
and LIPI11-2-Ac042
represent two novel species of the genus
, for which the names
sp. nov. (type strain LIPI11-2-Ac034
= InaCC A459
= NBRC 110973
) and
sp. nov. (type strain LIPI11-2-Ac042
= InaCC A458
= NBRC 110974
) are proposed. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1466-5026 1466-5034 |
| DOI: | 10.1099/ijsem.0.000499 |